教育背景
1987年9月-1992年7月北京师范大学生物化学专业,学士
1999年3月-2004年10月荷兰格罗宁根大学化学系,博士
工作经历
1992年7月-1996年9月中国科学院生物物理研究所细胞室,实习研究员
1996年9月-1999年3月中国科学院生物物理研究所细胞室,助理研究员
2004年7月-2006年6月荷兰格罗宁根大学化学系,博士后
2006年10月-2014年6月电子科技大学生命科学与技术学院,副教授
2014年7月-至今电子科技大学生命科学与技术学院,教授
项目课题经历
论文、成果、著作等
1. Wang, X., Han, S., Yang, Z., Tang, L.* (2015) Improvement of the thermostability and activity of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by engineering C-Terminal amino acids. J. Biotechnol., 212:92-98.
2. Tang, L.*, Liu, Y., Jiang, R., Zheng, Y., Zheng, K., Zheng, H. (2015) A high-throughput adrenaline test for the exploration of the catalytic potential of halohydrin dehalogenases in epoxide-ring opening reactions. Biotechnol. Appl. Biochem. 62:451-457.
3. Mikleuševic´, A., Hameršak, Z., Salopek-Sondi, B., Tang, L. Janssen, D.B., Majerić -Elenkov, M. (2015) Oxazolidinone synthesis through halohydrin dehalogenase catalyzed dynamic kinetic resolution. Adv. Synth. Catal. 357:1709-1714.
4. Wang X, Lin H, Zheng Y, Feng J, Yang Z, Tang L. *(2015) MDC-Analyzer-facilitated combinatorial strategy for improving the activity and stability of halohydrin dehalogenase from Agrobacterium radiobacter AD1. J Biotechnol. 206:1-7.
5. Guo, C., Chen, Y., Zheng, Y., Zhang, W., Tao, Y., Feng, J, Tang, L.*(2015) Exploring the enantioselective mechanism of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by iterative saturation mutagenesis. Appl. Environ. Microbiol. 2015 81(8):2919-26.
6. Schallmey, M., Jekel, P., Tang, L., Majerić Elenkov, M., Höffken, H.W., Hauer, B., Janssen, D.B. (2015) A single point mutation enhances hydroxynitrile synthesis by halohydrin dehalogenase. Enzyme Microb. Technol. 70:50-57.
7. Wang, X., Zheng, Z., Zheng, H., Nie, H., Yang, Z., and Tang, L.* (2014) DC-Analyzer-facilitated combinatorial strategy for rapid directed evolution of functional enzymes with multiple mutagenesis sites. J. Biotechnol. 192:102-107
8. Lixia Tang*, Yu Liu, Rongxiang Jiang, Yu Zheng, Kai Zheng, and Huayu Zheng. (2014) A high-throughput adrenaline test for the exploration of the catalytic potential of halohydrin dehalogenases in epoxide-ring opening reactions. Biotechnol. Appl. Biochem. doi: 10.1002/bab.1278.
9. Tang, L., Wang, X., Ru, B., Sun, H., Huang, J., and Gao, H. (2014) MDC-Analyzer: a novel degenerate primer design tool for the construction of intelligent mutagenesis libraries with contiguous sites. BioTechniques 56:301-310.
10. Tang, L*., Zheng, K., Liu, Y., Zheng, Z., Wang, H., Song, C., Zhou, H. (2013) Exploring the potential of megaprimer PCR in conjunction with orthogonal array design for mutagenesis library. Biotechnol. Appl. Biochem. 60:190-195.
11. Majerić Elenkov, M., Primožič, I., Hrenar, T., Smolko, A., Dokli, I., Salopek-Sondi, B., Tang, L. (2012) Catalytic activity of halohydrin dehalogenases towards spiroepoxides. Org. Biomol. Chem. 10:5063-5072.
12. Tang, L*., Gao, H., Zhu, X., Wang, X., Zhou, M. and Jiang, R. (2012) Construction of ‘small-intelligent’ focused mutagenesis libraries using well-designed combinatorial degenerate primers. BioTechniques 52:149-158.
13. Tang, L*., Zhu, X., Zheng, H., Jiang, R. and Majerić Elenkov, M. (2012) Key residues for controlling enantioselectivity of halohydrin dehalogenase from Arthrobacter sp. AD2 revealed by structure-guided directed evolution. Appl. Environ. Microb.78:2631-2637.
14. Tang, L*., Jiang, R., Zheng, K. and Zhu, X. (2011) Enhancing the recombinant protein expression of halohydrin dehalogenase HheA in Escherichia coli by applying a codon optimization strategy. Enzyme Microb.Technol., 49: 395-401.
15. Tang, L*., Li, L., and Wang, X. (2010) A high-throughput colorimetric assay for screening halohydrin dehalogenase saturation mutagenesis libraries. J. Biotechnol. 147, 164-168.
专利、著作版权等
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