1977年开始从事组织学与胚胎学专业。1980年攻读中山医学院硕士研究生 1988年攻读华西医科大学(现四川大学)博士研究生,研究吗啡对脊髓可塑性的影响

1998年在美国印第安纳州大学医学院做博士后研究 2003年在香港大学医学院做访问学者，研究成体干细胞的分化

共获35项校内、外科研基金, 其中有国家自然科学基金(11项)、国家重点研发计划项目课题、教育部博士学科点优先发展领域项目和广东省重点科技专项基金等。科研项目研究课题：

1. 神经元中继器修复全横断脊髓损伤的机制研究；国家自然科学基金重大项目课题(81891003)，负责人：曾园山，2019

2. 基于干细胞的神经组织模块构建及神经损伤修复研究；国家重点研发计划项目(2017YFA0104700)，负责人：丁斐，参与人：曾园山，2017

3. 三维明胶海绵支架材料在非人灵长类脊髓损伤治疗中的转化医学研究；广东省科技发展专项资金项目(2017B020210012)，负责人：曾园山，2017

4. 督脉电针促进干细胞源性神经元靶向性生长修复脊髓损伤的机制研究；国家自然科学基金面上项目 (81674064)，负责人：曾园山，2016

5. NSC源性神经网络支架移植修复脊髓受损伤神经网络的实验研究；教育部博士学科点优先发展领域项目(201300193035)，负责人：曾园山，2013

6. 靶向调节内源性神经干细胞分化促进脊髓损伤后移植神经网络与宿主功能性突触连接；国家自然科学基金联合基金项目(U1301223)，负责人：沈慧勇，参与人：曾园山，2013

7. 干细胞源性神经网络支架移植修复脊髓受损伤神经网络的机制研究；国家自然科学基金重点项目(81330028)，负责人：曾园山，2013

8. Fig4 基因对神经损伤修复的影响机制研究--延续项目；国家自然科学基金海外学者合作项目(81129019)，负责人：李俊/曾园山，2011

9. 督脉电针与基因修饰MSCs移植联合应用治疗多发性硬化的实验研究；国家自然科学基金面上项目 (30973721)，负责人：曾园山，2009

10. Fig4 基因对神经损伤修复的影响机制研究；国家自然科学基金海外学者合作项目(30828016)，负责人：李俊/曾园山，2008

11. TrkC基因修饰MSCs分化移植促进脊髓损伤修复的机制及应用研究；国家自然科学基金面上项目 (30771143)，负责人：曾园山，2007

自1984年至现在, 发表学术论文171余篇, 其中65篇发表在国际学术期刊上 2001年主编《人体结构学》, 94.7万字, 北京: 科学出版社出版；
2001年主编《组织学胚胎学(考试辅导丛书)》, 33.0万字, 北京: 科技文献出版社出版；
2002年主编《组织学与胚胎学实验指导》, 20.0万字, 北京: 科技文献出版社出版；
2004年主编《组织学胚胎学(考试辅导教材)》修订版, 47.7万字, 北京: 科技文献出版社出版；
2004年主编《组织学和胚胎学》, 30.0万字, 北京: 科学出版社出版；
2006年主编《组织学胚胎学考点》, 37.6万字, 北京: 科技文献出版社出版；
2009年主编《组织学胚胎学导学与应试指南》, 30.9万字, 北京: 科技文献出版社出版；
2010年主编《组织学和胚胎学(全国医药高等学校规划教材)》, 25.0万字, 北京: 科学出版社出版；
2011年主编《组织学与胚胎学实验指导》, 11.5万字, 北京: 中国医药科技出版社出版；
2012年主编《A Laboratory Manual of Histology and Embryology, 组织学与胚胎学实验指导(英文版)》, 19.4万字, 北京: 人民卫生出版社；
2014年主编《组织学与胚胎学实验指导》，14.6万字，北京：人民卫生出版社出版；
2015年主编临床医学《组织学与胚胎学(数字化规划教材)》，国家电子书包，北京: 人民军医出版社出版；
2017年主编临床医学《组织学与胚胎学(第9版全国规划教材)》, 北京: 人民卫生出版社出版。



2007年副主编《发育神经生物学》, 北京: 科学出版社出版；

2012年副主编《再生医学(全国高等医学院校教材)》, 北京: 人民卫生出版社出版；

2013年副主编5年制临床医学《组织学与胚胎学(第8版全国规划教材)》, 北京: 人民卫生出版社出版；

2015年副主编8年制临床医学《组织学与胚胎学(第3版全国规划教材)》, 北京: 人民卫生出版社出版；

2017年副主编《再生医学(全国高等医学院校教材)》, 北京: 人民卫生出版社出版。 Lai BQ, Che MT, Feng B, Bai YR, Li G, Ma YH, Wang LJ, Huang MY, Wang YQ, Jiang B, Ding Y, Zeng X*, Zeng YS*. Tissue-engineered neural network graft relays excitatory signal in the completely transected canine spinal cord. Advanced Science, 2019, 6(22):1901240 (1 of 19). Huang LJ, Li G, Ding Y, Sun JH, Wu TT, Zhao W, Zeng YS*. LINGO-1 deficiency promotes nerve regeneration through reduction of cell apoptosis, inflammation, and glial scar after spinal cord injury in mice. Exp Neurol, 2019, 320:112965. Jin H, Zhang YT, Yang Y, Wen LY, Wang JH, Xu HY, Lai BQ, Feng B, Che MT, Qiu XC, Li ZL, Wang LJ, Ruan JW, Jiang B, Zeng X, Deng QW, Li G, Ding Y*, Zeng YS*. Electroacupuncture facilitates the integration of neural stem cell-derived neural network with transected rat spinal cord. Stem Cell Reports, 2019, 12(2):274-289. Wu GH, Shi HJ, Che MT, Huang MY, Wei QS, Feng B, Ma YH, Wang LJ, Jiang B, Wang YQ, Han I, Ling EA, Zeng X*, Zeng YS*. Recovery of paralyzed limb motor function in canine with complete spinal cord injury following implantation of MSC-derived neural network tissue. Biomaterials, 2018, 181:15-34. Lai BQ, Feng B, Che MT, Wang LJ, Cai S, Huang MY, Gu HY, Jiang B, Ling EA, Li M, Zeng X*, Zeng YS*. A modular assembly of spinal cord-like tissue endows targeted tissue repair in the transected spinal cord. Advanced Science, 2018, 5(9):1800261(1 of 14). Li G, Che MT, Zeng X, Qiu CX, Feng B, Lai BQ, Shen HY, Ling EA, Zeng YS*. Neurotrophin-3 released from implant of tissue-engineered fibroin scaffolds inhibits inflammation, enhances nerve fiber regeneration and improves motor function in canine spinal cord injury. J Biomed Mater Res A, 2018, 106(8):2158-2170. Ma YH, Zeng X*, Qiu XC, Wei QS, Che MT, Ding Y, Liu Z, Wu GH, Sun JH, Pang M, Rong LM, Liu B, Aljuboori Z, Han I, Ling EA, Zeng YS*. Perineurium-like sheath derived from long-term surviving mesenchymal stem cells confers nerve protection to the injured spinal cord. Biomaterials, 2018, 160:37-55. Zou JL, Liu S, Sun JH, Yang WH, Xu YW, Rao ZL, Jiang B, Zhu QT, Liu XL, Wu JL, Chang C, Mao HQ, Ling EA, Quan DP*, Zeng YS*. Peripheral nerve-derived matrix hydrogel promotes remyelination and inhibits synapse formation. Advanced Functional Materials, 2018, 1705739 (1 of 12). Wang JM, Qu ZQ, Wu JL, Chung P, Zeng YS*. Mitochondrial protective and anti-apoptosis effects of Rhodiola Crenulata extract on hippocampal neurons in a rat model of Alzheimer’s disease. Neural Regen Res, 2017, 12(12):2025-2034. Zhang YT, Jin H, Wang JH, Wen LY, Yang Y, Ruan JW, Zhang SX, Ling EA, Ding Y*, Zeng YS*. Tail nerve electrical stimulation and electro-acupuncture can protect spinal motor neurons and alleviate muscle atrophy after spinal cord transection in rats. Neural Plast, 2017, 2017:7351238 (1 of 11). Yang XH, Ding Y, Li W, Zhang RY, Wu JL, Ling EA, Wu W, Zeng YS*. Effects of electroacupuncture and the RXR signaling pathway on oligodendrocyte differentiation in the demyelinated spinal cord of rats. Acupunct Med, 2017, 35(2):122-132. Lai BQ, Che MT, Du BL, Zeng X, Ma YM, Feng B, Qiu XC, Zhang K, Liu S, Shen HY, Wu JL, Ling EA, Zeng YS*. Transplantation of tissue engineering neural network and formation of neuronal relay into the transected rat spinal cord. Biomaterials, 2016, 109:40-54. Lin XY, Lai BQ, Zeng X, Che MT, Ling EA, Wu W, Zeng YS*. Cell transplantation and neuroengineering approach for spinal cord injury treatment: A summary of current laboratory findings and review of literature (an invited review). Cell Transplant, 2016, 25(8):1425-1438. Zeng X, Ma YH, Chen YF, Qiu XC, Wu JL, Ling EA, Zeng YS*. Autocrine fibronectin from differentiating mesenchymal stem cells induces the neurite elongation in vitro and promotes nerve fiber regeneration in transected spinal cord injury. J Biomed Mater Res A, 2016, 104(8):1902-1911. Li G, Che MT, Zhang K, Qin LN, Zhang YT, Chen RQ, Rong LM, Liu S, Ding Y, Shen HY, Long SM, Wu JL, Ling EA, Zeng YS*. Graft of the NT-3 persistent delivery gelatin sponge scaffold promotes axon regeneration, attenuates inflammation, and induces cell migration in rat and canine with spinal cord injury. Biomaterials, 2016, 83:233-248. Lai BQ, Qiu XC, Zhang K, Zhang RY, Jin H, Li G, Shen HY, Wu JL, Ling EA, Zeng YS*. Cholera toxin B subunit shows transneuronal tracing after injection in an injured sciatic nerve. PLoS One, 2015, 10(12):e0144030 (1 of 13). Qiu XC, Jin H, Zhang RY, Ding Y, Zeng X, Lai BQ, Ling EA, Wu JL, Zeng YS*. Donor mesenchymal stem cell-derived neural-like cells transdifferentiate into myelin-forming cells and promote axon regeneration in rat spinal cord transection. Stem Cell Res Ther, 2015, 6(1):105 (1 of 17). Zou J, Hu B, Arpag S, Yan Q, Hamilton A, Zeng YS, Vanoye CG, Li J. Reactivation of lysosomal Ca2+ efflux rescues abnormal lysosomal storage in FIG4-deficient cells. J Neurosci, 2015, 35(17):6801-6812. Ding Y, Zhang RY, He B, Liu Z, Zhang K, Ruan JW*, Ling EA, Wu JL, Zeng YS*. Combination of electroacupuncture and grafted mesenchymal stem cells overexpressing TrkC improves remyelination and function in demyelinated spinal cord of rats. Scientific Reports, 2015, 5:9133 (1 of 14). Zeng X, Qiu XC, Ma YH, Duan JJ, Chen YF, Gu HY, Wang JM, Ling EA, Wu JL, Wu WT, Zeng YS*. Integration of donor mesenchymal stem cell-derived neuron-like cells into host neural network after rat spinal cord transection. Biomaterials, 2015, 53:184-201. Du BL, Zeng X, Ma YH, Lai BQ, Wang JM, Ling EA, Wu JL, Zeng YS*. Graft of the gelatin sponge scaffold containing genetically modified neural stem cells promotes cell differentiation, axon regeneration and functional recovery in rat with spinal cord transection. J Biomed Mater Res A, 2015, 103(4):1533-1545. Liu Z, He B, Zhang RY, Zhang K, Ding Y, Ruan JW, Ling EA, Wu JL, Zeng YS*. Electroacupuncture promotes the differentiation of transplanted bone marrow mesenchymal stem cells pre-induced with neurotrophin-3 and retinoic acid into oligodendrocyte-like cells in demyelinated spinal cord of rats. Cell Transplant, 2015, 24(7):1265-1281. Guo JS*, Qian CH, Ling EA, Zeng YS*. Nanofiber scaffolds for treatment of spinal cord injury. Curr Med Chem, 2014, 21(37):4282-4289. Zhang K, Liu Z, Li G, Lai BQ, Qin LN, Ding Y, Ruan JW, Zhang SX, Zeng YS*. Electro-acupuncture promotes the survival and differentiation of transplanted bone marrow mesenchymal stem cells pre-induced with neurotrophin-3 and retinoic acid in gelatin sponge scaffold after rat spinal cord transection. Stem Cell Rev Rep, 2014, 10(4):612-625. Du BL, Zeng CG, Zhang W, Quan DP, Ling EA, Zeng YS*. A comparative study of gelatin sponge scaffolds and PLGA scaffolds transplanted to completely transected spinal cord of rat. J Biomed Mater Res A, 2014, 102(6):1715-1725. Lai BQ, Wang JM, Ling EA, Wu JL, Zeng YS*. Graft of a tissue engineered neural scaffold serves as a promising strategy to restore myelination after rat spinal cord transection. Stem Cells Dev, 2014, 23(8):910-921. Chen YF, Zeng X, Zhang K, Lai BQ, Ling EA, Zeng YS*. Neurotrophin-3 stimulates migration of mesenchymal stem cells overexpressing TrkC. Curr Med Chem, 2013, 20(24): 3022-3033. Lai BQ, Wang JW, Duan JJ, Chen YF, Gu HY, Ling EA, Wu JL, Zeng YS*. The integration of NSC-derived and host neural networks after rat spinal cord transection. Biomaterials, 2013, 34(12):2888-2901. Ding Y, Yan Q, Ruan JW, Zhang YQ, Li WJ, Zeng X, Huang SF, Zhang YJ, Wu JL, Fisher D, Dong H*, Zeng YS*. Electroacupuncture promotes the differentiation of transplanted bone marrow mesenchymal stem cells overexpressing TrkC into neuron-like cells in transected spinal cord of rats. Cell Transplant, 2013, 22(1):65-86. Li WJ, Li SM, Ding Y, He B, Keegan J, Dong H, Ruan JW*, Zeng YS*. Electro-acupuncture upregulates CGRP expression after rat spinal cord transection. Neurochem Int, 2012, 61(8):1397-1403. Zhou Y, Qu ZQ, Zeng YS*, Lin YK, Li Y, Chung P, Wong R, Hägg U. Neuroprotective effect of preadministration with Ganoderma lucidum spore on rat hippocampus. Exp Toxicol Pathol, 2012, 64:673-680. Zhang YQ, He LM, Xing B, Zeng X, Zeng CG, Zhang W, Quan DP, Zeng YS*. Neurotrophin-3 gene modified Schwann cells promote TrkC gene modified mesenchymal stem cells to differentiate into neuron-like cells in PLGA multiple-channel conduit. Cells Tissues Organs, 2012, 199(4):313-322. Ma YH, Zeng X, Zhang K, Zeng YS*. A new in vitro injury model of mouse neurons induced by mechanical scratching. Neurosci Lett, 2012, 510(1):14-19. Zhang YJ, Zhang W, Lin CG, Ding Y, Huang SF, Wu JL, Li Y, Dong H, Zeng YS*. Neurotrophin-3 gene modified mesenchymal stem cells promote remyelination and functional recovery in the demyelinated spinal cord of rats. J Neurol Sci, 2012, 313(1-2):64-74. Qu ZQ, Zhou Y, Zeng YS*, Lin YK, Li Y, Zhong ZQ, Chan WY*. Protective effects of a rhodiola crenulata extract and salidroside on hippocampal neurogenesis against streptozotocin-induced neural injury in the rat. PLoS One, 2012, 7(1):e29641. Guo JS, Ma YH, Yan Q, Wang LL, Zeng YS*, Wu JL, Li Jun*. Fig4 expression in the nervous system and its protection against abnormal lysosomal accumulation. J Neuropath Exp Neurol, 2012, 71(1):28-39. Zeng X, Zeng YS*, Ma YH, Lu LY, Du BL, Zhang W, Li Y, Chan WY*. Bone marrow mesenchymal stem cells in a three dimensional gelatin sponge scaffold attenuate inflammation, promote angiogenesis and reduce cavity formation in experimental spinal cord injury. Cell Transplant, 2011, 20(11/12):1881-1899. Liu Z, Ding Y, Zeng YS*. A new combined therapeutic strategy of Governor Vessel electro-acupuncture and adult stem cell transplantation promotes the recovery of injured spinal cord (an invited review). Curr Med Chem, 2011, 18:5165-5171. Ding Y, Yan Q, Ruan JW, Zhang YQ, Li WJ, Zeng X, Huang SF, Zhang YJ, Wang S, Dong H, Zeng YS*. Bone marrow mesenchymal stem cells and electro-acupuncture downregulate the inhibitor molecules and promote the axonal regeneration in the transected spinal cord of rats. Cell Transplant, 2011, 20(4):475-491. Wang JM, Zeng YS*, Wu JL, Li Yan, Teng YD*. Cograft of Schwann cells and neural stem cells overexpressing neurotrophin-3 and TrkC respectively after rat spinal cord transection. Biomaterials, 2011, 32(30):7454-7468. Du BL, Xiong Y, Zeng CG, He LM, Zhang W, Quan DP, Wu JL, Li Y, Zeng YS*. Transplantation of artificial neural construct partly improved spinal tissue repair and functional recovery in rats with spinal cord transection. Brain Res, 2011, 1400:87-98. Huang SF, Ding Y, Ruan JW, Zhang W, Wu JL, He B, Zhang YJ, Li Y, Zeng YS*. An experimental electro-acupuncture study in treatment of the rat demyelinated spinal cord injury induced by ethidium bromide. Neurosci Res, 2011,70:294-304. Yan Q, Ruan JW, Ding Y, Li WJ, Li Y, Zeng YS*. Electro-acupuncture promotes differentiation of mesenchymal stem cells, regeneration of nerve fibers and partial functional recovery after spinal cord injury. Exp Toxicol Pathol, 2011, 63:151-156. Zhang W, Yan Q, Zeng YS*, Zhang XB, Xiong Y, Wang JM, Chen SJ, Li Y, Bruce IC, Wu WT*. Implantation of adult bone marrow-derived mesenchymal stem cells transfected with the neurotrophin-3 gene and pretreated with retinoic acid in completely transected spinal cord. Brain Res, 2010, 1358:256-271. Li WJ, Pan SQ, Zeng YS*, Su BG, Li SM, Ding Y, Li Y, Ruan JW*. Identification of acupuncture-specific proteins in the process of electro-acupuncture after spinal cord injury. Neurosci Res, 2010, 67:307-316. Zhang YQ, Zeng X, He LM, Ding Y, Li Yan, Zeng YS*. NT-3 gene modified Schwann cells promote TrkC gene modified mesenchymal stem cells to differentiate into neuron-like cells in vitro. Anat Scie Inter, 2010, 85(2):61-67. Ding Y, Yan Q, Ruan JW, Zhang YQ, Li WJ, Zhang YJ, Li Y, Dong H, Zeng YS*. Electro-acupuncture promotes survival, differentiation of the bone marrow mesenchymal stem cells as well as functional recovery in the spinal cord-transected rats. BMC Neurosci, 2009, 10(1):35-47. Chen QG, Zeng YS*, Qu ZQ, Tang JY, Qin YJ, Chung P, Wong R, Hägg U. The effects of Rhodiola rosea extract on 5-HT level, cell proliferation and quantity of neurons at cerebral hippocampus of depressive rats. Phytomedicine, 2009, 16(9):830-838. Xiong Y, Zeng YS*, Zeng CG, Du BL , He LM, Quan DP, Zhang W, Wang JM, Wu JL, Li Y, Li J. Synaptic transmission of neural stem cells seeded in 3-dimensional PLGA scaffolds. Biomaterials, 2009, 30: 3711-3722. Zhang W, Zeng YS*, Wang JM, Ding Y, Li Y, Wu W. Neurotrophin-3 improves retinoic acid-induced neural differentiation of skin-derived precursors through a p75NTR-dependent signaling pathway. Neurosci Res, 2009, 64(2): 170-176. Qu ZQ, Zhou Y, Zeng YS* , Li Y, Chung P. Pretreatment of Rhodiola Rosea extract can reduce cognitive impairment induced by intracerebroventricular streptozotocin in rats: implication to anti-oxidative and neuroprotective effects. Biomed Environ Sci, 2009, 22(4):318-326. Tang JY，Zeng YS*，Peter C，Wong R，Urban H. Effects of Valeriana officinalis extract on rat depression model. Journal of Chinese Clinical Medicine, 2008, 3(7):374-378. Chen YY，Zhang W，Chen YL，Chen SJ，Dong H，Zeng YS*. Electro-acupuncture improves survival and migration of transplanted neural stem cells in injured spinal cord in rats. Acupunct Electrother Res, 2008, 33( 1-2 ):19-31. Guo J, Su H, Zeng YS, Liang YX, Wong WM, Ellis-Behnke RG, So KF, Wu WT. Reknitting the injured spinal cord by self-assembling peptide nanofiber scaffold. Nanomedicine, 2007, 3(4):311-321. Zhang XB, Zeng YS*, Zhang W, Wang JM, Wu JL, Li J. Co-transplantation of neural stem cells and NT-3-overexpressing Schwann cells in transected spinal cord. J Neurotrauma, 2007, 24(12):1863-1877. Guo JS, Zeng YS*, Li HB, Huang WL, Liu RY, Li XB, Ding Y, Wu LZ, Cai DZ. Cotransplant of neural stem cells and NT-3 gene modified Schwann cells promote the recovery of transected spinal cord injury. Spinal Cord, 2007, 45(1):15-24. Wang JM, Zeng YS*, Liu RY, Huang WL, Xiong Y, Wang YH, Chen SJ, Teng YD*. Recombinant adenovirus vector-mediated functional expression of neurotropin-3 receptor (TrkC) in neural stem cells. Exp Neurol, 2007, 203(1):123-127. Zeng YS, Nie JH, Zhang Wei, Chen SJ, Wu WT. Morphine acts via µ-opioid receptors to enhance spinal regeneration and synaptic reconstruction of primary afferent fibers injured by sciatic nerve crush. Brain Res, 2007,1113:108-113. Zhang W, Zeng YS*, Zhang XB, Wang JM, Zhang W, Chen SJ. Combination of adenoviral vector-mediated neurotrophin-3 gene transfer and retinoic acid promotes adult bone marrow cells to differentiate into neuronal phenotypes. Neurosci Lett, 2006, 408(2):98-103. Zhang XB, Zeng YS*, Zhang W, Chen YY, Zhang W, Xiong Y, Chen SJ. Synergistic effect of Schwann cells and retinoic acid on the neuronal differentiation and synaptogenesis of hippocampal neural stem cells in vitro. Biomed Environ Sci, 2006, 19(3):219-224. Zeng YS, Ding Y, Wu LZ, Guo JS, Li HB, Wong WM, Wu WT. Co-transplantation of Schwann cells promotes the survival and differentiation of neural stem cells transplanted into the injured spinal cord. Dev Neurosci, 2005, 27(1):20-26. Zeng YS, Xu Z C. Co-existence of necrosis and apoptosis in rat hippocampus following transient forebrain ischemia. Neurosci Res, 2000, 37(2)：113-125.

申请和授权专利情况：
1. 曾园山, 李 戈, 孙佳慧。Method for preparation of extracellular matrix-modified decellularized nerve scaffold and use thereof. 美国发明专利，专利申请号: US 15/730,770

2. 曾园山, 李 戈。一种具有趋化功能的生物活性支架的制备和应用。获中华人民共和国发明专利, 于2018年5月4日授权，发明专利号: ZL 201510252317.9

3. 曾园山, 李戈, 孙佳慧。一种基质化去细胞神经支架。获中华人民共和国实用新型专利, 于2018年1月30日授权，实用新型专利号: ZL 201621132126.5

4. 曾园山, 曾 湘。一种用于修复脊髓损伤的人工神经网络样导管的构建。获中华人民共和国发明专利, 于2015年11月6日授权，发明专利号: ZL 201010506133.8

5. 曾园山, 李 戈。一种具有趋化功能的生物活性支架的制备和应用。获中华人民共和国实用新型专利, 于2015年12月9日授权，实用新型专利号: ZL 201520319632.4

6. 曾园山, 曾 湘。一种用于修复神经损伤的明胶海绵圆柱体支架的构建。获中华人民共和国发明专利, 于2011年6月8日授权，发明专利号: ZL 200910040176.9

7. 曾园山, 王俊梅。人神经营养素-3受体基因重组腺病毒构建方法。获中华人民共和国发明专利, 于2008年7月16日授权，发明专利号: ZL 200510033569.9