2000.09-2004.06 安徽师范大学 本科/学士 专业：生物科学

2013.09-2014.10 美国宾州州立大学 博士后

2010.11-2013.08 美国马里兰大学 博士后

2004.09-2010.06   中科院植物生理生态研究所   研究生/博士         专业：遗传学

1、 创制油菜突变体群体。利用CRISPR/Cas9基因编辑技术构建油菜突变体，目前已经获得了一百多个油菜激素合成和信号相关的突变体。在CRISPR编辑载体的设计、突变位点的分析、油菜遗传转化和功能验证方面建立了一套成熟的实验体系（Yang et al., 2017）。此外，通过改进现有的基因编辑载体，提高转基因阳性植株的筛选效率（Tang et al., 2018）。




2、 油菜自交不亲和信号通路的解析。自交不亲和是一种重要的自然现象，前人发现盐水可以打破甘蓝型油菜的自交不亲和。利用蛋白组，对授粉前后的油菜柱头差异表达的蛋白进行分析表明，盐水可以特异性诱导某些特异蛋白的表达，这为解析油菜自交不亲和信号通路奠定了基础（Yang et al., 2018）。MLPK是自交不亲和信号通路中的关键基因，对油菜MLPK同源拷贝基因的研究发现，BnaA3.MLPK是参与自交不亲和的主效基因（Yang et al., 2019）。




3、 酪蛋白激酶参与水稻开花时间和株型的调控。通过筛选水稻T-DNA插入突变群体获得了一系列的水稻早花突变体。对其中的一株突变体进行基因定位，发现T-DNA插入在一个编码水稻I型酪蛋白激酶基因EL1（early flowering1）的外显子上,导致基因缺失。基因表达水平检测证明外源施加赤霉素可以明显抑制EL1的表达；体外磷酸化实验证明，EL1通过磷酸化水稻DELLA家族蛋白SLR1影响其蛋白活性，调控水稻赤霉素信号通路，进而负调节水稻开花时间。该结果第一次发现了一个新的I型酪蛋白激酶基因可以磷酸化修饰水稻DELLA蛋白，证明了赤霉素信号通路在水稻中可以调控开花时间（Dai et al., 2010）。




4、 拟南芥钙调素蛋白调控叶片衰老机制。钙调素蛋白是生物体响应钙信号的重要蛋白。研究表明，拟南芥钙调素蛋白CaM1和CaM4与RPK1互作，调控活性氧在植物体内的积累水平调节叶片衰老（Koo et al., , 2017 and Dai et al., 2018）。

· Chen F, Yang Y, Li B, Liu Z, Khan F, Zhang T, Zhou G, Tu J, Shen J, Yi B, Fu T, Dai C*, Ma C*. Functional Analysis of M-Locus Protein Kinase Revealed a Novel Regulatory Mechanism of Self-Incompatibility in Brassica napus L. Int J Mol Sci. 2019 Jul 5;20(13). pii: E3303. doi: 10.3390/ijms20133303.

· Tang T, Yu X, Yang H, Gao Q, Ji H, Wang Y, Yan G, Peng Y, Luo H, Liu K, Li X, Ma C, Kang C, Dai C. Development and Validation of an Effective CRISPR/Cas9 Vector for Efficiently Isolating Positive Transformants and Transgene-Free Mutants in a Wide Range of Plant Species. Front Plant Sci. 2018 Oct 23;9:1533. doi: 10.3389/fpls.2018.01533. eCollection 2018.

· Feng J, Dai C, Luo H, Han Y, Liu Z, Kang C. Reporter gene expression reveals precise auxin synthesis sites during fruit and root development in the wild strawberry. J Exp Bot. 2018 Oct 27. doi: 10.1093/jxb/ery384.

· Dai C, Lee Y, Lee IC, Nam HG, Kwak JM. Calmodulin 1 Regulates Senescence and ABA Response in Arabidopsis. Front Plant Sci. 2018 Jul 2;9:803. doi: 10.3389/fpls.2018.00803. eCollection 2018.

· Yang Y, Liu Z, Zhang T, Zhou G, Duan Z, Li B, Dou S, Liang X, Tu J, Shen J, Yi B, Fu T, Dai C*, Ma C*. Mechanism of Salt-Induced Self-Compatibility Dissected by Comparative Proteomic Analysis in Brassica napus L. Int J Mol Sci. 2018 Jun 3; 19(6). pii: E1652. doi: 10.3390/ijms19061652.

· Luo H, Dai C, Li Y, Feng J, Liu Z, Kang C. Reduced Anthocyanins in Petioles codes for a GST anthocyanin transporter that is essential for the foliage and fruit coloration in strawberry. J Exp Bot. 2018 Apr 27;69(10):2595-2608. doi: 10.1093/jxb/ery096.

· Koo JC#, Lee IC#, Dai C#, Lee Y#, Cho HK, Kim Y, Phee BK, Kim H, Lee IH, Choi SH, Park SJ, Jeon IS, Nam HG, Kwak JM. The Protein Trio RPK1-CaM4-RbohF Mediates Transient Superoxide Production to Trigger Age-Dependent Cell Death in Arabidopsis. Cell Reports. 2017 Dec 19;21(12):3373-3380. doi: 10.1016/j.celrep.2017.11.077.

· Yang H, Wu JJ, Tang T, Liu KD*, Dai C*. CRISPR/Cas9-mediated genome editing efficiently creates specific mutations at multiple loci using one sgRNA in Brassica napus. Sci Rep. 2017 Aug 8;7(1):7489. doi: 10.1038/s41598-017-07871-9.

· Zhao B, Li H, Li J, Wang B, Dai C, Wang J, Liu K. Brassica napus DS-3, encoding a DELLA protein, negatively regulates stem elongation through gibberellin signaling pathway. Theor Appl Genet. 2017 Apr;130(4):727-741. doi: 10.1007/s00122-016-2846-4. Epub 2017 Jan 16.

· Li Y, Dai C, Hu C, Liu Z, Kang C. Global identification of alternative splicing via comparative analysis of SMRT- and Illumina-based RNA-seq in strawberry. Plant J. 2017 Apr;90(1):164-176. doi: 10.1111/tpj.13462. Epub 2017 Feb 11.

· Shih HW, Miller ND, Dai C, Spalding EP, Monshausen GB. The Receptor-like Kinase FERONIA Is Required for Mechanical Signal Transduction in Arabidopsis Seedlings. Current Biology. 2014 Aug 18;24(16):1887-92. doi: 10.1016/j.cub.2014.06.064.

· Tan ST, Dai C, Liu HT, Xue HW. Arabidopsis casein kinase1 proteins CK1.3 and CK1.4 phosphorylate cryptochrome2 to regulate blue light signaling. Plant Cell. 2013 Jul;25(7):2618-32. doi: 10.1105/tpc.113.114322.

· Wen BQ, Xing MQ, Zhang H, Dai C, Xue HW. Rice homeobox transcription factor HOX1a positively regulates gibberellin responses by directly suppressing EL1. Journal of Integrative Plant Biology. 09/2011; 53(11):869-78.
· Dai C, Xue HW. Rice EARLY FLOWERING1, a CKI, Phosphorylates DELLA Protein SLR1 to Negatively Regulate Gibberellin Signaling. The EMBO Journal. 06/2010; 29(11):1916-27.

会议报告：


· 2019年6月15TH INTERNATIONAL RAPESEED CONGRESS 口头报告 "CRISPR/Cas9-mediated genome editing efficiently creates specific mutations in Brassica napus." 德国 柏林

· 2017年12月 中国科协第338次青年科学家论坛-反向遗传学与基因功能研究 口头报告 "CRISPR/Cas9-mediated genome editing efficiently creates specific mutations at multiple loci using one sgRNA in Brassica napus." 中国 武汉

· 2014年6月Midwest Plant Cell Dynamics Annual Meeting口头报告 "Identification of proteins interacting with the receptor-like kinase FERONIA." 美国 威斯康辛

· 2013年7月ASPB Plant Biology Annual Meeting. Poster "Production and Endocytosis of Superoxide Endosomes Trigger Age-Dependent Cell Death in Arabidopsis". 美国 罗德岛

· 2011年4月Mid-Atlantic Section ASPB Annual Spring Meeting. 口头报告 "Effects and functional mechanism of rice EL1, a casein kinase I, in gibberellin acid signaling." 美国 马里兰

· 2008年11月6th International Conference on the Frontiers of Plant Biology. 中国 丽江

· 2005年10月1st International Conference on the Frontiers of Plant Biology. 中国 上海