领域：生物产业 学校：复旦大学职称：
膜蛋白例如离子通道，转运蛋白，在多种细胞信号传递过程中起着至关重要的作用，上市的一半以上药物的靶点为膜蛋白。因此，高分辨率的膜蛋白的三维结构不仅在基础科学层面上，而且在药物研究层面上都具有极大意义。然而，膜蛋白结构解析时所需要的表达，纯化技术是非常困难的，因此大多数的膜蛋白，特别是来自真核生物的膜蛋白的三维结构还不明瞭。但是最近这几年，由于膜蛋白结构研究技术的发展，膜蛋白的结构解析逐渐成为生命科学界中非常热门的领域。
本实验室的研究兴趣是膜蛋白的分子机制的原子级别的理解，特别是那些与人类疾病相关的离子通道以及它们的调节蛋白。为了实现以上目标，我们利用了X射线晶体学得到目标蛋白的三维结构，然后通过电生理学以及生化方法的组合进行结构引导的突变分析。
本实验室非常欢迎对膜蛋白结构感兴趣的本科生，研究生，博士后以及研究助理的加入。本实验室的导师会根据实验室每位成员对研究课题以及今后职业道路的期望，与实验室的每位成员探讨最合适的研究课题。此外，进入本实验室，可以学习使用最先进的仪器进行膜蛋白结构生物学研究的最前沿技术。这些技术不仅是在基础研究层面，如今在制药公司等产业界也得到了极大的重视。此外，本实验室与美国以及日本的许多实验室都有长期密切合作关系。实验室的成员将有机会前往国外进行实验以及参加国际会议。对于将来有计划前往国外攻读学位或者从事博士后研究的同学，也是非常适合加入本实验室的。
Membrane proteins such as ion channels and transporters play a crucial role in various cell signaling processes, and represent more than half of targets for commercially available drugs. Accordingly, high-resolution 3D structures of membrane proteins are of great interest at the level of both basic science and drug discovery research. The Hattori Lab is interested in atomic-level understanding of the molecular mechanism of membrane proteins, particularly of those associated with human diseases. To achieve these goals, we employ X-ray crystallography to obtain 3D structures of our target membrane proteins, and then conduct structure-guided mutational analysis by a combination of electrophysiological and biochemical approaches. Open postdoc and student positions are currently available....
具体了解该专家信息，请致电：027-87555799 邮箱 haizhi@uipplus.com
1. Jia Y, Zhao Y, Kusakizako T, Wang Y, Pan C, Zhang Y, Nureki O*, Hattori M*, Yan Z*." TMC1 and TMC2 Proteins Are Pore-Forming Subunits of Mechanosensitive Ion Channels." Neuron. 2019,DOI:10.1016/j.neuron.2019.10.017. *Corresponding authors.
2. Li M, Wang Y, Banerjee R, Marinelli F, Silberberg S, Faraldo-Gomez J*, Hattori M*, Swartz K*."Molecular mechanisms of human P2X3 receptor channel activation and modulation by divalent cation bound ATP." eLife. 2019, 8:e47060. *Corresponding authors.
3. Wang J, Wang Y, Cui WW, Huang Y, Yang Y, Liu Y, Zhao WS, Cheng XY, Sun WS, Cao P, Zhu MX, Wang R*, Hattori M*, Yu Y*."Druggable negative allosteric site of P2X3 receptors." Proc Natl Acad Sci U S A. 2018, 115(19):4939-4944. *Corresponding authors.
4. Kasuya G, Yamaura T, Ma XB, Nakamura R, Takemoto M, Nagumo H, Tanaka E, Dohmae N, Nakane T, Yu Y, Ishitani R, Matsuzaki O*, Hattori M*, Nureki O*. "Structural insights into the competitive inhibition of the ATP-gated P2X receptor channel." Nat Commun. 2017, 8(1):876. *Corresponding authors.
5. Tomita A, Zhang M, Jin F, Zhuang W, Takeda H, Maruyama T, Osawa M, Hashimoto KI, Kawasaki H, Ito K, Dohmae N, Ishitani R, Shimada I, Yan Z*, Hattori M*, Nureki O*. "ATP-dependent modulation of MgtE in Mg2+ homeostasis." Nat Commun. 2017, 8(1):148. *Corresponding authors.
6. Kasuya G, Hiraizumi M, Maturana AD, Kumazaki K, Fujiwara Y, Liu K, Nakada-Nakura Y, Iwata S, Tsukada K, Komori T, Uemura S, Goto Y, Nakane T, Takemoto M, Kato HE, Yamashita K, Wada M, Ito K, Ishitani R, Hattori M*, Nureki O*. "Crystal structures of the TRIC trimeric intracellular cation channel orthologues." Cell Res. 2016, (12):1288-1301. *Corresponding authors.
7. Kasuya G, Fujiwara Y, Takemoto M, Dohmae N, Nakada-Nakura Y, Ishitani R, Hattori M*, Nureki O*. "Structural Insights into Divalent Cation Modulations of ATP-Gated P2X Receptor Channels." Cell Rep. 2016, 14(4):932-944. *Corresponding authors.
8. Hattori M and Gouaux E., “Molecular mechanism of ATP binding and ion channel activation P2X receptors.” Nature 2012, 485(7397) 207-212.
9. Hattori M, Tanaka Y, Fukai S, Ishitani R, Nureki O., “Crystal structure of the MgtE Mg2+ transporter.”Nature 2007, 448(7157) 1072-1075.
