领域：生物产业 学校：南京大学职称：教授
生化与分子药理学、细胞生物学、生物化学与分子生物学
1：钠钾ATP酶及其配体的新生物学功能研究Na,K-ATPase is a ubiquitous ion pump across membrane, in recent years, increasing evidence reveals that besides ion-pumping activity, this ion transporter is also actively involved in many patho-physiological regulation via multiple signaling pathway. Furthermore, the endogenous Na,K-ATPase inhibitors are separated and identified from human serum or urine, but the biological function of these active components remains elusive. Our current project is to explore the novel biological function of Na,K-ATPase as well as its inhibitors in cancer or inflammation.
2：炎症因子mRNA转录后水平调控机制及其在药物筛选中的应用价值The post-transcriptional regulation of inflammatory cytokines plays a critical role in a variety of immune regulation, which allows the cells to elaborately control the progression of immune reaction. Many strategies are adopted by cells to regulate cytokines mRNA turnover, such as miRNA or RNA binding proteins (RBPs).Among them, we pay special attention to RBPs such as HuR, and try to uncover the novel function of these RBPs in controlling cytokines mRNA turnover. We are also interested in the special intercellular organelles that are responsible for mRNA reprogramming. Based on these mechanisms, we expect to screen anti-inflammatory herbal medicine extracts , and to identify new chemicals and novel mechanisms. 3：小分子抗肿瘤或增敏剂活性物质的寻找与机制研究It has long been recognized that not all cancer cells are sensitive to a certain chemotherapeutic drug, such as As2O3. In view of this deficiency, to increase tumor cells sensitivity towards anti-tumor drugs has become a heat topic. We show great interest in looking for small molecules that can significant increase cancer cells sensitivity towards tumor-targeting drugs from the library of the nature products, and expect to identify the promising drug target for cancer therapy. 4: 中药抗肿瘤分子药理学The herbal medicine has made great contribution to the discovery of new anti-cancer drug. Recently, many chemicals for herbal medicine have shown significant anti-tumor effect, but the molecular mechanisms remain not fully understood. On this project, we expect to identify the mechanisms of some of these natural products by multiple molecular as well as cellular approaches.
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具体了解该专家信息，请致电：027-87555799 邮箱 haizhi@uipplus.com
论文
1. Cao D, Bian JJ, Hua ZC, Ma L, Chen W, Cheng S, Sun W, Jiao QC, and Yin W*. Modulation of TNF-a mRNA Stability by Human Antigen R and miR181s in Sepsis-Induced Immunoparalysis. EMBO mol Med. 2014 DOI - 10.15252/emmm.201404797 (5 year IF: 9.186)
2. Yin W, Cheng W, Shen W, Shu L, Zhao J, Zhang J, Hua ZC. Impairment of Na(+),K(+)-ATPase in CD95(APO-1)-induced human T-cell leukemia cell apoptosis mediated by glutathione depletion and generation of hydrogen peroxide. Leukemia. 2007; 21: 1669-78.(IF: 10.132)
3. Yin, W, Sonia, D., & Zhou, X. H2O2 is necessary but not sufficient to the stimulatory effect of low K on the Na,K-ATPase. J. Am. Soc. Nephrol. 2002;13:482. (IF:9.466)
4. Feng S, Chen W, Cao D, Gong FY, Cheng W, Chen S, Xu Q, Hua ZC, Yin W*.(2011) Involvement of Na,K-ATPase and its inhibitors in HuR-mediated cytokine mRNA stabilization in lung epithelial cells. Cell & Mol. Life Sci 68（1）109-24 (IF:7.1)
5.Shi YL, Feng S, Chen W, Hua ZC, Bian JJ, Yin W*.Mitochondrial inhibitor sensitizes non-small-cell lung carcinoma cells to TRAIL-induced apoptosis by reactive oxygen species and Bcl-XL/p53-mediated amplification mechanisms. Cell Death Dis. 2014 Dec 18;5:e1579. doi: 10.1038/cddis.2014.547. (IF: 6.1)
6. Chen W., Ma L, Feng S, Cao D, Hua ZC, and Yin W. General Control Non-Depressing Kinase 2 Generates Auto-Amplification Loop in Cancer Cell Apoptosis by Posttranslational Mechanisms. Molecular biology of the Cell. 2015 （accepted）(5-year IF: 5.1)
7. Yin W, Li X, Feng S, Cheng W, Tang B, Shi YL, Hua ZC. Plasma membrane depolarization and Na,K-ATPase impairment induced by mitochondrial toxins augment leukemia cell apoptosis via a novel mitochondrial amplification mechanism. Biochem Pharmacol. 2009; 78: 191-202. (IF:4.9)
8. Deng X, Yin F, Lu X, Cai B, Yin W*. The apoptotic effect of brucine from the seed of Strychnos nux-vomica on human hepatoma cells is mediated via Bcl-2 and Ca2+ involved mitochondrial pathway. Toxicol Sci. 2006; 91: 59-69.（IF:4.7)
9. Yin W*, Yin FZ, Shen WX, Cai BC, Hua ZC. Requirement of hydrogen peroxide and Sp1 in the stimulation of Na,K-ATPase by low potassium in MDCK epithelial cells. Int J Biochem Cell Biol. 2008; 40: 942-53. (IF:4.9)
10. Yin W*, Deng XK, Yin FZ, Zhang XC, Cai BC. The cytotoxicity induced by brucine from the seed of Strychnos nux-vomica proceeds via apoptosis and is mediated by cyclooxygenase 2 and caspase 3 in SMMC 7221 cells. Food Chem Toxicol. 2007; 45: 1700-8.
11. Yin W, Deng XK, Li WD, Yin FZ, Lu XY, Zhang XC, Hua ZC, Cai BC. The anti-tumor effects of alkaloids from the seeds of Strychnos nux-vomica on HepG2 cells and its possible mechanism. J Ethnopharmacol. 2006; 106: 179-86.
12. Yin W, Wang TS, Yin FZ, Cai BC. Analgesic and anti-inflammatory properties of brucine and brucine N-oxide extracted from seeds of Strychnos nux-vomica. J Ethnopharmacol. 2003; 88: 205-14.
13. Yin FZ#, Yin W#, Zhang X,Lu TL, Cai BC. Development of an HPLC fingerprint for quality control and species differentiation of Fructus schisandrae Acat chromatographia 2010;22(4):609-621. 共同第一作者
14. Yin W, Jiang G, Takeyasu K, Zhou X. Stimulation of Na,K-ATPase by low potassium is dependent on transferrin. J Membr Biol 2003;193:177-84.
15. Yin W, Cai BC. Role of transferrin in the stimulation of Na,K-ATPase induced by low K+ in Madin Darby canine kidney cells. Acta. Physiol. Sin. 2003; 55: 481-6. (IF:0.6)
16. Yin, W, Yin, FZ., & Cai BC, The mechanism of cytotoxicity of human embryonic kidney cells induced by brucine. Chin. J. Pharmacol. & Toxicol. 2003,17(5):321-327
17. Yin FZ, Lu TL, Cai BC, and Yin W*. Development of a quality control method for Schisandrae chinensis fructus with micellar electrokinetic capillary chromatography. Acta pol. Pharm. 2014 accepted.
18. Yin FZ, Lu TL, Li L, Cai BC, and Yin W*. Quality control of Gardeniae Fructus by HPLC-PDA fingerprint coupled with chemometric methods. Journal of chromatographic science. 2014 accepted
19. Shu L, Yin W, Zhang J, Tang B, Kang YX, Ding F, Hua ZC. Sinomenine inhibits primary CD4+ T-cell proliferation via apoptosis. Cell Biol Int. 2007; 31: 784-9.
20. Li S, Dong W, Zong Y, Yin W, Jin G, Hu Q, Huang X, Jiang W, Hua ZC. Polyethylenimine-complexed plasmid particles targeting focal adhesion kinase function as melanoma tumor therapeutics. Mol Ther. 2007; 15: 515-23.
专著
《酶的凝胶电泳检测手册》化学工业出版社 编委
《药食本草》中国中医药科技出版社 副主编
《基因工程实验创新教材》 知识出版社 主编
专利
1. ZL201010235528.9，第一发明人
2. ZL20100242655.1，第一发明人
3. ZL201010241247.4，第一发明人
4．蟾蜍甾二烯类化合物在制备治疗脓毒症免疫麻痹药物中的用途,201310693592.2，第一发明人
5．强心甾类化合物在制备治疗脓毒症免疫麻痹药物中的用途，201310597606.3 ，第一发明人
6．miR181s在制备治疗急性肠炎药物中的应用，201310654116.2，第一发明人
7．一种用于治疗脓毒症急性肺损伤的小核酸药物，201310652876.X ，第一发明人
8．以NLRP3为靶点的药物筛选细胞模型及其应用，201310695899.9 ，第一发明人
9．他克莫斯FK506在制备治疗非小细胞肺癌药物中的应用，201310674818.7，第一发明人
10．一种五味子有机酸分析方法，201310654107.3，第二发明人
11．4-氧代戊酸在制备止泻药物中的用途，201310654108.8，第二发明人
