教育背景
1982, B.S., 南京农业大学,植物保护学学士
1985, M.S., 南京农业大学,植物病理学硕士
1985 -1989,南京师范大学,生物系讲师
1993, Ph.D., 美国佐治亚大学,微生物学系,理学博士
工作经历
1994 -1996, 威斯康辛大学、加州大学,博士后
1997 -1999, 美国Scripps医学研究所,副研究员
2000.03-2003.02, 江南大学分子生物学特聘教授,太湖学者
2002.07-2012.06, 南京师范大学生物化学/分子生物学,特聘教授
2012.04起,江苏大学环境科学,特聘教授
项目课题经历
近5年主持的主要项目
■ 乙醇发酵细菌中木糖转运和代谢系统的分子重组研究,国家自然科学基金,2008-2010
■ 一株嗜冷菌及镶嵌其细胞壁的大型颗粒的研究,国家自然科学基金,2010-2012
■ 反馈抑制在嗜热细菌乙醇代谢关键酶的基因转录中的发生过程及作用机理,国家自然科学基金,2012-2015
论文、成果、著作等
近5年发表SCI收录文章20余篇
代表性论文和专利:
1. Qian G, Chen C, Zhou R, He Y, Shao W*. 2014. A thermostable S-adenosylhomocysteine hydrolase from Thermotoga maritima: Properties and its application on S-adenosylhomocysteine production with enzymatic cofactor regeneration. Enzyme Microb Technol, 64-65: 33-37.
2. Wang H, Wang H, Wang M, Zhang L, Wang R, Mei Y, Shao W*. 2014. Identification and refinement of two strong constitutive promoters for gene expression system of Schizosaccharomyces pombe. World J Microb Biotechnol, 30: 1809-1817.
3. Le Y, Chen H, Zagursky R, Wu JHD*, Shao W*.¬¬ 2013. Thermostable DNA ligase-mediated PCR production of circular plasmid (PPCP) and its application in directed evolution via in situ error-prone PCR. DNA Res, doi:10.1093/dnares/dst016. (IF 5.164)
4. Huang C-H, Sun Y, Ko T-P, Chen C-C, Zheng Y, Chan H-C, Pang X, Wiegel J*, Shao W*, and Guo R-T*. 2012. The substrate/product-binding modes of a novel GH120 β-xylosidase (XylC) from Thermoanaerobacterium saccharolyticum JW/SL-YS485. Biochem J, 448: 401–407. (IF 4.897)
5. Zheng Z, Li H, Li L, Shao W*. 2012. Biobleaching of wheat straw pulp with recombinant laccase from the hyperthermophilic Thermus thermophilus HB27. Biotechnol Lett, 34: 541–547. (IF 1.768)
6. Mei Y, Sun Y, He J, Wang Q, Sun Y, Shao W*. 2012. Genome Sequences of Pseudomonas fragi Strains A22 and B25. J Bacteriol, 194(12):3276. (IF 3.825)
7. Qu W, Shao W*. 2011. Cloning, expression and characterization of glycoside hydrolase family 11 endoxylanase from Bacillus pumilus ARA. Biotechnol Lett. 33: 1407-1416. (IF 1.768)
8. Le Y, Peng J, Wu H, Sun J, Shao W*. 2011. A technique for production of soluble protein from a fungal gene encoding an aggregation-prone xylanase in Escherichia coli. PLoS ONE, 6(4): e18489. (IF 4.092)
9. Wu G, Sun Y, Qu W, Huang Y, Lu L, Li L, Shao W*. 2011. Application of GFAT as a novel selection marker to mediate gene expression. PLoS ONE, 6(2): e17082. (IF 4.092)
10. Pei J, Zhou Q, Jing Q, Li L, Dai C, Li H, Wiegel J, Shao W*. 2011. The mechanism for regulating ethanol fermentation by Redox levels in Thermoanaerobacter ethanolicus. Metab Eng, 13: 186–193. (IF 5.614)
11. Shao W, Xue Y, Wu A, Kataeva I, Pei J, Wu H, Wiegel J*. 2011. Characterization of a novel beta-xylosidase XylC from Thermoanaerobacterium saccharolyticum JW/SL-YS485. Appl. Environ. Microbiol, 77(3): 719-726. (IF 3.829)
12. Pei J, Zhou Q, Jiang Y, Le Y, Li H, Peng H, Shao W*, Wiegel J. 2010. Thermoanaerobacter spp control ethanol pathway via transcriptional regulation and versatility of key enzymes. Metab Eng, 12: 420–428. (IF 5.614)
13. Wu H, Pei J, Jiang Y, Song X, Shao W*. 2010. pHsh vectors, a novel expression system of Escherichia coli for the large-scale production of recombinant enzymes. Biotechnol Lett, 32:795–801 (IF 1.768)
14. Le Y, Peng J, Pei J, Li H, Duan Z, Shao W*. 2010. Properties of an NAD+-dependent DNA ligase from the hyperthermophile Thermotoga maritima and its application in PCR amplification of long DNA fragments. Enzyme Microb Technol, 46: 113–117. (IF 2.367)
15. Peng H, Wu G, Shao W *. 2008. The aldehyde/alcohol dehydrogenase (AdhE) in relation to the ethanol formation in Thermoanaerobacter ethanolicus JW200. Anaerobe, 14 (2): 125-127. (IF 2.409)
16. Jiang Y, Song X, Gong L, Li P, Dai C, Shao W*. 2008. High poly (-hydroxybutyrate) production by Pseudomonas fluorescens A2a5 from inexpensive substrates. Enzyme Microb Technol, 42: 167-172. (IF 2.367)
17. Wu H, Pei J, Wu G, Shao W*. 2008. Overexpression of GH10 endoxylanase XynB from T. maritima in E. coli by a novel vector with potential for industrial application. Enzyme Microb Technol, 42: 230-234. (IF 2.367)
18. Pei J, Shao W*. 2008. Purification and characterization of an extracellular -L-arabinosidase from a novel isolate Bacillus pumilus ARA and its over-expression in Escherichia coli. Appl Microbiol Biotechnol, 78: 115-121. (IF 3.425)
19. Jang Y, Pei J, Song X, Shao W *. 2007. Restriction site-dependent PCR: an efficient technique for cloning of new genes from genomic DNA. DNA Res, 14: 283-290. (IF 5.164)
20. Zeng H, Xue Y, Peng T, Shao W*. 2007. Properties of xylanolytic enzyme system in bifidobacteria and their effects on the utilization of xylooligosaccharides Food Chemistry, 101: 1172-1177. (IF 3.655)
专利、著作版权等
申请国际发明专利2项、国家发明专利16项,已获得1项美国专利授权和13项中国发明专利授权。
国际专利PCT/CN2004/000742、PCT/US2010/027364;
美国授权专利US7,807,460B2;
中国发明专利授权:ZL2003131524.0、ZL200410065776.8、ZL200410064667.4、ZL200410044977.X、ZL200510039239.0、ZL200510038237.X、ZL200610096305.2、
ZL200610040383.0、ZL200710020315.2、ZL 2008 1 0018772.2、ZL200910025925.0、ZL 201010567825.3
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