1999-2003 河南工业大学，食品科学与工程专业，工科学士 2003-2004 罗牛山股份有限公司，技术员 2005-2007 江南大学，食品科学与工程专业，工学硕士 2007-2011 江南大学，发酵工程，工学博士

2012-2016 江南大学，生物工程学院，讲师 2016-现在 江南大学，生物工程学院，副教授，硕士生导师 其中： 2012-2012 日本大阪大学，工学院，访问学者 2019-2020 日本理化学研究所，可持续资源科学中心，访问学者

1、 基于蛋白质折叠调控的链霉菌谷氨酰胺转胺酶高效分泌表达研究. 国家自然科学基金面上项目 (31771913) . 主持 2、 融合自组装双亲短肽提高Pseudomonas aeruginosa脂肪氧合酶热稳定性机制的研究. 国家自然科学 基金青年基金项目(31401638). 主持 3、甾体激素产品高效提取工艺及优化.国家重点研发计划子课题(2019YFA0905304).主持 4、合成塑料高效生物降解的关键技术. 国家重点研发计划子课题(2019YFA0706900). 主持 5、 调控Streptomyces hygroscopicus 谷氨酰胺转胺酶折叠速率强化异源分泌的研究. 江苏省自然科学 基金青年基金项目(BK20130132). 主持 6、 有机酸生物合成途径构建与优化技术. 863子课题(2015AA021003). 主持 7、 耐热脂肪氧合酶高效制备关键技术. 江苏省重点研发计划社会发展项目(BE2016629). 参与

SCI论文 1、Zhao WX, Du GC, Zhou JW, Liu S*. 2019. An expression tag library based on self-assembling amphipathic peptides for overproduction of recombinant proteins. Microbial Cell Factories 18:91 2、Feng, Y, Liu S*, Jiao Y, Wang Y, Wang M; Du G. 2019. Gene cloning and expression of the Lasparaginase from Bacillus cereus BDRD-ST26 in Bacillus subtilis WB600. Journal of Bioscience and Bioengineering 127(4): 418-424. 3、Feng Y, Liu S*, Pang C, Gao H, Wang M, Du G. 2019. Improvement of catalytic efficiency and thermal stability of l-asparaginase from Bacillus subtilis 168 through reducing the flexibility of the highly flexible loop at N-terminus. Process Biochemistry 78: 42-49. 4、Zhao W, Du G, Liu S*. An efficient thermostabilization strategy based on self-assembling amphipathic peptides for fusion tags. 2019. Enzyme and Microbial Technology 121: 68–77 5、Zhao W, Liu L*, Du G, Liu S*. 2018. A multifunctional tag with the ability to benefit the expression, purification, thermostability and activity of recombinant proteins. Journal of Biotechnology 283:1–10 6、Feng Y, Liu S*, Jiao Y, Wang Y, Wang M, Du G, Chen J*. 2018. Improvement of L-asparaginase thermal stability by regulating enzyme kinetic and thermodynamic states. Process Biochemistry 71: 45-52 7、Feng Y, Liu S*, Jiao Y, Gao H, Wang M, Du G, Chen J*. 2017. Enhanced extracellular production of Lasparaginase from Bacillus subtilis 168 by B-subtilis WB600 through a combined strategy. Applied Microbiology and Biotechnology 101:1509-1520. 8、Lu X, Wang G, Feng Y, Liu S*, Zhou X, Du G, Chen J. 2016. The N-Terminal alpha-Helix Domain of Pseudomonas aeruginosa Lipoxygenase Is Required for Its Soluble Expression in Escherichia coli but Not for Catalysis. Journal of Microbiology and Biotechnology 26:1701-1707. 9、Liu S, Wang M, Du G, Chen J. 2016. Improving the active expression of transglutaminase in Streptomyces lividans by promoter engineering and codon optimization. BMC Biotechnology 16. 10、Bi J, Liu S, Du G, Chen J. 2016. Bile salt tolerance of Lactococcus lactis is enhanced by expression of bile salt hydrolase thereby producing less bile acid in the cells. Biotechnology Letters 38:659-665. 11、Liu S, Wan D, Wang M, Madzak C, Du G, Chen J. 2015. Overproduction of pro-transglutaminase from Streptomyces hygroscopicus in Yarrowia lipolytica and its biochemical characterization. BMC Biotechnology 15. 12、Liu S, Dai J, Kang Z, Li J, Chen J, Du G. 2015. Production of novel NaN3-resistant creatine amidinohydrolase in recombinant Escherichia coli (vol 6, pg 248, 2015). Bioengineered 6:248-250. 13、Xu Z, Liu S*, Lu X, Rao S, Kang Z, Li J, Wang M, Chen J*. 2014. Thermal inactivation of a recombinant lipoxygenase from Pseudomonas aeruginosa BBE in the absence and presence of additives. Journal of the Science of Food and Agriculture 94:1753-1757. 14、Lu X, Liu S*, Feng Y, Rao S, Zhou X, Wang M, Du G*, Chen J. 2014. Enhanced thermal stability of Pseudomonas aeruginosa lipoxygenase through modification of two highly flexible regions. Applied Microbiology and Biotechnology 98:1663-1669. 15、Lu X, Zhang J, Liu S, Zhang D, Xu Z, Wu J, Li J, Du G, Chen J. 2013. Overproduction, purification, and characterization of extracellular lipoxygenase of Pseudomonas aeruginosa in Escherichia coli. Applied Microbiology and Biotechnology 97:5793-5800. 16、Lu X, Liu S, Zhang D, Zhou X, Wang M, Liu Y, Wu J, Du G, Chen J. 2013. Enhanced thermal stability and specific activity of Pseudomonas aeruginosa lipoxygenase by fusing with self-assembling amphipathic peptides. Applied Microbiology and Biotechnology 97:9419-9427.17、Chen K, Zhang D, Liu S, Wang NS, Wang M, Du G, Chen J. 2013. Improvement of transglutaminase production by extending differentiation phase of Streptomyces hygroscopicus: mechanism and application. Applied Microbiology and Biotechnology 97:7711-7719. 18、Chen K, Liu S*, Zhang D, Wang M, Shi Z, Du G, Chen J*. 2013. Altered secretary efficiency of Streptomyces hygroscopicus transglutaminase in Escherichia coli by the pro-peptide modification. Process Biochemistry 48:782-787. 19、Chen K, Liu S, Wang G, Zhang D, Du G, Chen J, Shi Z. 2013. Enhancement of Streptomyces transglutaminase activity and pro-peptide cleavage efficiency by introducing linker peptide in the Cterminus of the pro-peptide. Journal of Industrial Microbiology & Biotechnology 40:317-325. 20、Yang Y, Zhang D, Liu S, Jia D, Du G, Chen J. 2012. Expression and fermentation optimization of oxidized polyvinyl alcohol hydrolase in E. coli. Journal of Industrial Microbiology & Biotechnology 39:99104. 21、Chen K, Liu S, Ma J, Zhang D, Shi Z, Du G, Chen J. 2012. Deletion combined with saturation mutagenesis of N-terminal residues in transglutaminase from Streptomyces hygroscopicus results in enhanced activity and thermostability. Process Biochemistry 47:2329-2334. 22、Liu S, Zhang D, Wang M, Cui W, Chen K, Liu Y, Du G, Chen J, Zhou Z. 2011. The pro-region of Streptomyces hygroscopicus transglutaminase affects its secretion by Escherichia coli. FEMS Microbiology Letters 324:98-105. 23、Liu S, Zhang D, Wang M, Cui W, Chen K, Du G, Chen J, Zhou Z. 2011. The order of expression is a key factor in the production of active transglutaminase in Escherichia coli by co-expression with its propeptide. Microbial Cell Factories 10. 24、Liu S, Zhang D, Liu L, Wang M, Du G, Chen J. 2010. Enhanced water absorption of wheat gluten by hydrothermal treatment followed by microbial transglutaminase reaction. Journal of the Science of Food and Agriculture 90:658-663. 专著 1、Jian Chen, Yang Zhu, Song Liu. Functional Carbohydrates: Development, Characterization, and Biomanufacture. 2017, CRC Press, ISBN 9781498718776

1. Song Liu, Weixin Zhao, Jian Chen, Guocheng Du. Multi-functional peptide benefiting expression, purification, stabilization and catalytic efficiency of recombinant proteins. Patent No.: US10273266B2. 2. Song Liu, Jian Chen, Guocheng Du, Weixin Zhao. Asparaginase mutant with efficient expression, activity and stability. Patent No.: US10337018B1. 3. Song Liu, Yue Feng, Jian Chen, Guocheng Du, Yun Jiao, Jie Ruan, Hongye Cheng, Hui Gao. Mutant with enhanced secretion of L-asparaginase and its application. Patent No.: US9896678B2 4. Jian Chen, Guocheng Du, Xinyao Lu, Song Liu, Juan Zhang. Method for increasing protein thermal stability. Patent No.: US9040274B2 5. 刘松 陈坚 堵国成 赵伟欣 一种比酶活和热稳定性提高的碱性果胶酶突变体 专利号： 201510828863.2 6. 刘松 冯岳 陈坚 堵国成 陈双全 王广圣 陈璇 一种酶活提高的天冬酰胺酶突变体 专利号： 201410568323.0 7. 刘松 陈双全 任立均 陈坚 堵国成 一种产碱性果胶酶的重组菌及其应用 专利号：201610906975.X 8. 刘松 方芳 陈坚 堵国成 毕洁 一种增强乳酸菌胆汁耐受能力的方法 专利号：201510918010.8 9. 刘松 堵国成 陈坚 毕洁 一种底物专一性提高的胆盐水解酶突变体 专利号：2016100706080 10. 刘松 童理明 陈坚 堵国成 一种分泌量提高的谷氨酰胺转氨酶 专利号：2015110264547 11. 刘松 陈坚 堵国成 毕洁 一种产胆盐水解酶变异体的基因工程菌及其制备方法 专利号： 2016100274725 12. 陈坚 刘松 阮洁 冯岳 李江华 陈双全 邱芳芳 王晨蕾 一种热稳定性提高的肌酸水解酶突变体 专利 号：2015107667580 13. 刘松 方芳 陈坚 堵国成 毕洁 一种乳酸乳球菌胆汁耐受能力提高的胆盐水解酶变异体 专利号： 2015110320481 14. 刘松 阮洁 冯岳 陈坚 堵国成 黎清华 一种提高L-天冬酰胺酶分泌表达的方法 专利号： 2015101027326