2001-2005 河南农业大学，动物医学专业，学士 2005-2007 吉林大学， 生物化学与分子生物学专业，硕士 2007-2010 南开大学， 生物化学与分子生物学专业，博士

2010-2014 江南大学生物工程学院，讲师 2014-至今 江南大学生物工程学院，副教授，硕士生导师 2017-2018 康奈尔大学（美国），化学与生物分子工程学院，访问学者

1. Bacillus subtilis双精氨酸转运系统中信号肽定向识别的分子机制，国家自然科学基金 (31400058)，项目负责人 2. L-苯丙氨酸解氨酶定向进化及对映体选择识别机制的研究，江苏省自然科学基金 (BK20130139)，项目负责人 3. 重组枯草芽孢杆菌联产纳豆激酶和维生素K2的产物协同积累机制及发酵工艺开发，江南 大学自主科研-重点项目（JUSRP51713B），项目负责人 4. 高活性和高稳定性新型腈水合酶的开发和应用，国家重点研发计划-政府间国际科技创新 合作项目(2016YFE012740)，参与 3. 重组高分泌型枯草芽孢杆菌重组表达系统的开发与应用,863计划(SS2014AA021304)，参 与 4. 谷氨酰胺转胺酶的开发及高效制备，教育部科学技术研究重大项目(311023)，参与

[1] Han L, Chen Q, Lin Q, Cheng J, Zhou L, Liu Z, Guo J, Zhang L, Cui W*, Zhou Z*. Realization of Robustness and Precise Regulation of Gene Expression by Multiple Sigma Recognizable Artificial Promoters. Front in Bioeng Biotechnol. 2020, 8:92. [2] Han L, Cui W*, Suo F, Miao S, Hao W, Chen Q, Guo J, Liu Z, Zhou L, Zhou Z*.Development of a novel strategy for robust synthetic bacterial promoters based on a stepwise evolution targeting the spacer region of the core promoter in Bacillus subtilis. Microb Cell Fact. 2019; 18(1):96. [3] Cui W*, Han LC, Suo FY, Liu ZM, Zhou L, Zhou ZM*. Exploitation of Bacillus subtilis as a robust workhorse for production of heterologous proteins and beyond. World J Microbiol Biotechnol. 2018; 34: [4] Cui W*, Suo F, Cheng J, Han L, Hao W, Guo J, Zhou Z*. Stepwise modifications of genetic parts reinforce the secretory production of nattokinase in Bacillus subtilis. Microb Biotechnol 2018; 11: 930-942. [5] Lan Y, Zhang XH, Liu ZM, Zhou L, Shen RH, Zhong XP, Cui W*, Zhou ZM*. Overexpression and characterization of two types of nitrile hydratases from Rhodococcus rhodochrous J1. PLoS One 2017; 12: e0179833.[6] Han L, Suo FY, Jiang C, Gu J, Li NN, Zhang NX, Cui W*, Zhou ZM*. Fabrication and characterization of a robust and strong bacterial promoter from a semi-rationally engineered promoter library in Bacillus subtilis. Process Biochem. 2017; 61: 56-62. [7] Cui W, Cheng J, Miao S, Zhou L, Liu Z, Guo J, Zhou Z*. Comprehensive characterization of a theophylline riboswitch reveals two pivotal features of Shine-Dalgarno influencing activated translation property. Appl Microbiol Biotechnol 2017; 101: 2107-2120. [8] Cui W, Han L, Cheng J, Liu Z, Zhou L, Guo J, Zhou Z*. Engineering an inducible gene expression system for Bacillus subtilis from a strong constitutive promoter and a theophyllineactivated synthetic riboswitch. Microb Cell Fact 2016; 15: 199. [9]Guan C, Cui W*, Cheng J, Zhou L, Liu Z, Zhou Z*. Development of an efficient autoinducible expression system by promoter engineering in Bacillus subtilis. Microb Cell Fact 2016; 15: 66. [10] Guan C, Cui W, Cheng J, Liu R, Liu Z, Zhou L, Zhou Z*. Construction of a highly active secretory expression system via an engineered dual promoter and a highly efficient signal peptide in Bacillus subtilis. N Biotechnol 2016; 33: 372-379.

1、国内发明专利 一种来源于放线菌的腈水合酶基因在大肠杆菌中高效表达的方法，ZL201210345154.5，授 权 一种生产高光学纯度D-苯丙氨酸的方法，ZL 201410364770.4，授权 一种强启动子及其在提高纳豆激酶表达的应用，201610004239.5，授权 2、国际发明专利 A fused NHase with improved specific activity and stability，14870023.0，授权