2020.01-至今：江南大学产业技术研究院，院长 2017.4-2019.12：江南大学生物工程学院，副院长 2014.10-至今: 江南大学生物工程学院，教授、博士生导师 2012.3-2013.3: 美国佐治亚理工学院，博士后 2010.8-2014.9: 江南大学生物工程学院，副教授 2009.5-2010.7: 江南大学生物工程学院，讲师

[1]国家重点研发计划项目：高版本模式微生物底盘细胞(2018YFA0900300，2019-2024 年) [2]国家重点研发计划课题：高版本大宗有机酸生产的丝状真菌底盘细胞构建(2018YFA0900504，2019-2024 年) [3]国家自然基金重点项目：枯草芽孢杆菌中母乳寡糖代谢网络的模块优化与动态调控(31930085，2020-2024 年) [4]国家优秀青年科学基金项目：微生物遗传育种(31622001, 2017-2019 年) [5]国家自然基金面上项目：由 glmS 核酶介导的枯草芽孢杆菌中 N-乙酰氨糖代谢网络的动态优化调控 (21676119, 2017-2020 年) [6]国家自然基金面上项目：基于枯草芽孢杆菌 Spo0F-Spo0A 双组分群体感应系统的维生素 K2 动态代谢调控 (31871784，2019-2022 年) [7]国家自然基金面上项目：克拉酸生物合成过程机制解析与代谢调控研究(31870069，2019-2022 年) [8]国家自然基金面上项目：基于 GlcNAc6P 特异性磷酸酶定向筛选与进化的 N-乙酰氨糖生物合成研究(31671845, 2017-2020 年) [9]国家自然基金青年基金：基于枯草芽孢杆菌功能膜微域支架的 N-乙酰氨基葡萄糖代谢调控研究(21808084， 2019-2021 年) [10]江南大学自主科研计划重点项目重组枯草芽孢杆菌联产纳豆激酶和维生素 K2 的产物协同积累机制及发酵工艺开 发(JUSRP51713B，2017-2020)

以第一作者或通讯作者在包括 Nucleic Acids Research、Nature Communications、Trends in Biotechnology Metabolic Engineering、ACS Synthetic Biology、Biotechnology and Bioengineering 等工业生物技术领域国际权威 学术期刊发表 SCI 论文 100 余篇。作为主编出版 2 部英文著作和 3 部中文著作，参与 5 部英文著作和 4 部本科生教 材的编写，部分代表性论著如下。 1、 Liu Long, Chen Jian. 《Systems and Synthetic Biotechnology for Production of Nutraceuticals》(Springer-Nature 出版 社)，2019 年, ISBN: 978-981-15-0445-7. 2、 Liu Long, Du Guocheng, Liu Yanfeng. 《Systems and Synthetic Metabolic Engineering》(Elsevier 出版社). 2020 年. ISBN: 978-0-12-821753-5. 3、 刘龙，陈坚，石贵阳，李由然. 《新一代柠檬酸发酵技术》. 化学工业出版社. 2018 年. ISBN：978-7-122-33643-9. 4、Wu Yaokang, Chen Taichi, Liu Yanfeng, Tian Rongzhen, Lv Xueqin, Li Jianghua, Du Guocheng, Chen Jian, Ledesma - Amaro Rodrigo, Liu Long*. Design of a programmable biosensor-CRISPRi genetic circuits for dynamic and autonomous dual-control of metabolic flux in Bacillus subtilis. Nucleic Acids Research. 2020. 48: 996- 1009. 5、 Xu Xianhao, Liu Yanfeng, Du Guocheng, Ledesma-Amaro Rodrigo, Liu Long*. Microbial chassis development for natural products biosynthesis. Trends in Biotechnology. 2020. doi: 10.1016/j.tibtech. 2020.01.002. 6、 Lv Xueqin, Wu Yaokang, Tian Rongzhen, Gu Yang, Liu Yanfeng, Li Jianghua, Du Guocheng, Ledesma-Amaro Rodrigo, Liu Long*. Synthetic metabolic channel by functional membrane microdomains for compartmentalized flux control. Metabolic Engineering. 2020. doi: 10.1016/j.ymben.2020.02.003. 7、 Lv Xueqin, Jin Ke, Wu Yaokang, Zhang Cheng, Cui Shixiu, Zhu Xiaonan, Li Jianghua, Du Guocheng, Liu Long*. Enzyme assembly guided by SPFH-induced functional inclusion bodies for enhanced cascade biocatalysis. Biotechnology and Bioengineering. 2020. doi: 10.1002/bit.27304 8、 Deng Jieying, Chen Chunmei, Gu Yang, Lv Xueqin, Liu Yanfeng, Li Jianghua, Ledesma-Amaro Rodrigo, Du Guocheng, Liu Long*. Creating an in vivo bifunctional gene expression circuit through an aptamer-based regulatory mechanism for dynamic metabolic engineering in Bacillus subtilis. Metabolic Engineering. 2019. 55: 179-190. 9、 Gu Yang, Liu Yanfeng, Li Jianghua, Du Guocheng, Ledesma-Amaro Rodrigo, Liu Long*. Synthetic redesign of central carbon and redox metabolism for high yield production of N-acetylglucosamine in Bacillus subtilis. Metabolic Engineering. 2019. 51: 59-69. 10、 Deng Jieying, Gu Liuyan, Chen Taichi, Huang Hao, Yin Xiaoqiang, Lv Xueqin, Liu Yanfeng, Li Nan, Liu Zhenmin, Li Jianghua, Du Guocheng, Liu Long*. Engineering the substrate transport and cofactor regeneration systems for enhancing 2’-fucosyllactose synthesis in Bacillus subtilis. ACS Synthetic Biology. 2019. 8: 2418-2427. 11、 Wu Yaokang, Chen Taichi, Liu Yenfeng, Lv Xueqin, Li Jianghua, Du Guocheng, Ledesma-Amaro Rodrigo, Liu Long*. CRISPRi allows optimal temporal control of N-acetylglucosamine bioproduction by a dynamic coordination of glucose and xylose metabolism in Bacillus subtilis. Metabolic Engineering. 2018. 49: 232-241. 12、 Gu Yang, Xu Xianhao, Wu Yaokang, Niu Tengfei, Liu Yanfeng, Li Jianghua, Du Guocheng, Liu Long*. Advances and prospects of Bacillus subtilis cellular factories: from rational design to industrial applications. Metabolic Engineering. 2018. 50: 109-121. 13、 Cui Shixiu, Lv Xueqin, Wu Yaokang, Li Jianghua, Du Guocheng, Ledesma-Amaro Rodrigo, Liu Long*. Engineering a bifunctional Phr60-Rap60-Spo0A quorum-sensing molecular switch for dynamic fine-tuning of menaquinone-7 synthesis in Bacillus subtilis. ACS Synthetic Biology. 2019. 8: 1826-1837. 14 Deng Jieying, Gu Liuyan, Chen Taichi, Huang Hao, Yin Xiaoqiang, Lv Xueqin, Liu Yanfeng, Li Nan, Liu Zhenmin, Li Jianghua, Du Guocheng, Liu Long*. Engineering the substrate transport and cofactor regeneration systems for enhancing 2’-fucosyllactose synthesis in Bacillus subtilis. ACS Synthetic Biology. 2019. 8: 2418-2427. 15、 Niu Tengfei, Liu Yanfeng, Li Jianghua, Koffas Matheson, Du Guocheng, Alper Hal S., Liu Long*. Engineering a glucosamine-6-phosphate responsive glmS ribozyme switch enables dynamic control of metabolic flux in Bacillus subtilis for overproduction of N-acetylglucosamine. ACS Synthetic Biology. 2018. 7: 2423-2435 16、Deng Chen, Li Jianghua, Liu Yanfeng, Du Guocheng, Ledesma-Amaro Rodrigo, Liu Long*. Synthetic repetitive extragenic palindromic (REP) sequence as an efficient mRNA stabilizer for protein production and metabolic engineering in prokaryotic cells. Biotechnology and Bioengineering. 2019. 116: 5-18 17、Yuan Haibo, Liu Yanfeng, Li Jianghua, Shin Hyun-dong, Du Guocheng, Shi Zhongping, Chen Jian, Liu Long*. Combinatorial synthetic pathway fine-tuning and comparative transcriptomics for metabolic engineering of Raoultella ornithinolytica BF60 to efficiently synthesize 2,5-furandicarboxylic acid. Biotechnology and Bioengineering. 2018. 115: 2148-2155 18、Guan Ningzi, Du Bin, Li Jianghua, Shin Hyun-dong, Chen Rachel R., Du Guocheng, Chen Jian, Liu Long*. Comparative genomics and transcriptomics analysis-guided metabolic engineering of Propionibacterium acidipropionici for improved propionic acid production. Biotechnology and Bioengineering. 2018. 115: 483-494. 19、Liu Yanfeng, Li Jianghua, Du Guocheng, Chen Jian, Liu Long*. Metabolic engineering of Bacillus subtilis fueled by systems biology: recent advances and future directions. Biotechnology Advances. 2017. 35: 20-30 20、Liu Yanfeng, Link Hannes, Liu Long*, Du Guocheng, Chen Jian*, Sauer Uwe. A novel dynamic pathway analysis approach reveals a limiting futile cycle in N -acetylglucosamine overproducing Bacillus subtilis. Nature Communications. 2016. 7:11933

获得中国授权发明专利 40 项，美国授权发明专利 8 项。美国授权发明专利如下： [1]Liu Long, Chen Jian, Du Guocheng, Li Jianghua, Niu Tengfei. Recombinant Bacillus subtilis for producing acetylglucosamine and construction method thereof. 授权专利号：US 10, 093, 936. [2]Liu Long, Chen Jian, Du Guocheng, Yin Xian, Li Jianghua. Promoter and use thereof. 授权专利号：US 9,926,569. [3]Liu Long, Chen Jian, Du Guocheng, Li Jianghua, Liu Yanfeng, Hannes Link, Uwe Sauer. Method for enhancing Nacetylglucosamine production through glck knockout of Bacillus subtilis. 授权专利号：US 9,914,949. [4]Liu Long, Chen Jian, Du Guocheng, Yin Xian, Li Jianghua. Method of gene expression regulation. 授权专利号：US 2018/0171344. [5]Liu Long, Chen Jian, Du Guocheng, Li Jianghua, Gu Yang, Song Yang, Deng Jieying, Zhao Yawen. Method for improving GlcNAc production of recombinant Bacillus subtilis. 授权专利号：US 9,868,970. [6]Chen Jian, Du Guocheng, Liu Long, Li Jianghua, Guan Ningzi. Method for improving acid tolerance of Propionibacterium acidipropionici. 授权专利号：US 9,187,773. [7]Chen Jian, Du Guocheng, Li Jianghua, Liu Long, Feng Zhuolin. Method for increasing microbial catalase production 授权专利号：US 8,722,364. [8]Liu Long, Li Jianghua, Niu Tengfei, Chen Jian, Du Guocheng. Recombinant Bacillus subtilis for increasing production of acetylglucosamine and construction method thereof. 授权专利号：US 10, 465, 198.