1988.8-1992.7　天津商学院食品系　 大学本科　获学士学位 1998年4月-2003年3月 日本筑波大学 应用生物化学系五年一贯制博士课程 获博士学位

2002年4月-2006年3月 日本学术振兴会特别研究员（JSPS） 2006年4月-2007年3月 日本筑波大学生命环境科学研究科 COE研究员 2007年4月-2009年8月 日本筑波大学生命环境科学研究科 博士后研究员 2009年9月- 江南大学生物工程学院 太湖学者特聘教授

主持或完成863计划、国家自然科学基金、江苏省产学研项目等多项，其中代表性的科 研项目如下: 1. 863课题， 2014AA021304、重组高分泌型枯草芽孢杆菌重组表达系统的开发与应用 、315万元、主持。 2. 江苏“双创”团队计划，创新型微生物外源蛋白质高效表达体系的研发、66万元、骨 干。 3. 国家外专高专人才项目，GDW20123200114、腈水合酶的生物合成及应用开发、200 万元、主持。 4. 教育部重大项目，311023、谷氨酰胺转胺酶的开发及高效制备、100万元、主持。 5. 国家自然科学基金，31070711、工业用腈水合酶全新蛋白质翻译后调节体系selfsubunit swapping的研究、35万元、主持。 6. 教育部新世纪优秀人才项目，丙烯酰胺生产用酶--腈水合酶的研究、50万元、主持 。 7. 江苏省科技厅产学研项目，纳豆激酶的开发与高效制备、15万元、主持。 8. 企业合作项目，生物法丙烯酰胺制备技术改造、50万元、主持。 9. 企业合作项目，纳豆激酶的开发与高效制备、75万元、主持。

1. Wenjing Cui*, Feiya Suo, Jintao Cheng, Laichuang Han, Wenliang Hao, Junling Guo, Zhemin Zhou*. Stepwise modifications of genetic parts reinforce the secretory production of nattokinase in Bacillus subtilis. Microb Biotechnol 11:930-942. 2. Wenjing Cui*, Laichuang Han, Feiya Suo, Zhongmei Liu, Li Zhou, Zhemin Zhou*.Exploitation of Bacillus subtilis as a robust workhorse for production of heterologous proteins and beyond. World Journal of Microbiology and Biotechnology (2018) 34:145. 3. Jinfeng Wang, Zhongmei Liu, Zhemin Zhou* .Cloning and Characterization of a Novel Thermophilic Amylopullulanase with a Type I Pullulanase Structure From Anoxybacillus sp WB42. Starch-Starke 70. doi:ARTN 1700265.10.1002/star.201700265 4. Jinfeng Wang, Zhongmei Liu, Zhemin Zhou* .The N-Terminal Domain of the Pullulanase from Anoxybacillus sp WB42 Modulates Enzyme Specificity and Thermostability. Chembiochem 19:949-955 5.Cheng Z, Cui W, Xia Y, Peplowski L, Kobayashi M, Zhou Z*: Modulation of NHase regioselectivity towards dinitriles by tailoring the substrate binding pocket residues. ChemCatChem 2017, In press. 6. Cheng Z, Peplowski L, Cui W, Xia Y, Liu Z, Zhang J, Kobayashi M, Zhou Z*: Identification of key residues modulating the stereoselectivity of nitrile hydratase toward racmandelonitrile by semi-rational engineering. Biotechnol Bioeng 2017. In press 7. Zhou L, Cui WJ, Liu ZM, Zhou ZM*: Metabolic engineering strategies for D-lactate over production in Escherichia coli. Journal of Chemical Technology and Biotechnology 2016, 91:576-584.8. Xia Y, Cui W, Liu Z, Zhou L, Cui Y, Kobayashi M, Zhou Z*: Construction of a subunitfusion nitrile hydratase and discovery of an innovative metal ion transfer pattern. Sci Rep 2016, 6:19183. 9. Guan C, Cui W, Cheng J, Zhou L, Liu Z, Zhou Z*: Development of an efficient autoinducible expression system by promoter engineering in Bacillus subtilis. Microb Cell Fact 2016, 15:66. 10. Cui W, Han L, Cheng J, Liu Z, Zhou L, Guo J, Zhou Z*: Engineering an inducible gene expression system for Bacillus subtilis from a strong constitutive promoter and a theophyllineactivated synthetic riboswitch. Microb Cell Fact 2016, 15:199. 11. Cheng ZY, Cui WJ, Liu ZM, Zhou L, Wang M, Kobayashi M, Zhou ZM*: A switch in a substrate tunnel for directing regioselectivity of nitrile hydratases towards alpha,omegadinitriles. Catalysis Science & Technology 2016, 6:1292-1296. 12. Guan C, Cui W, Cheng J, Zhou L, Guo J, Hu X, Xiao G, Zhou Z*: Construction and development of an auto-regulatory gene expression system in Bacillus subtilis. Microb Cell Fact 2015, 14:150. 13. Cui Y, Cui W, Liu Z, Zhou L, Kobayashi M, Zhou Z*: Improvement of stability of nitrile hydratase via protein fragment swapping. Biochem Biophys Res Commun 2014, 450:401-408. 14. Gao X, Cui W, Ding N, Liu Z, Tian Y, Zhou Z*: Structure-based approach to alter the substrate specificity of Bacillus subtilis aminopeptidase. Prion 2013, 7. 15. Zhou Z*, Hashimoto Y, Kobayashi M: Self-subunit swapping chaperone needed for the maturation of multimeric metalloenzyme nitrile hydratase by a subunit exchange mechanism also carries out the oxidation of the metal ligand cysteine residues and insertion of cobalt. J Biol Chem 2009, 284:14930-14938. 16. Zhou Z*, Hashimoto Y, Shiraki K, Kobayashi M: Discovery of posttranslational maturation by self-subunit swapping. Proc Natl Acad Sci U S A 2008, 105:14849-14854.

授权国际发明专利2项，国内发明专利8项。 A fused NHase with improved specific activity and stability 9896679 A fused NHase with improved specific activity and stability 9670474 一种强启动子及其在提高纳豆激酶表达的应用 ZL201610004239.5 一种新型启动子及其应用 ZL 201510203088.1 一种生产高光学纯度D-苯丙氨酸的方法 ZL201410364770.4 一种比酶活和稳定性提高的融合型腈水合酶 ZL201510195795.0 一种来源于放线菌的腈水合酶基因在大肠杆菌中高效表达的方法 ZL 201210345154.5 一种新型谷氨酰胺转胺酶及其应用 ZL 201010582053.0 一种谷氨酰胺转胺酶的分离纯化方法 ZL 201010580955.0 一种高效表达高分子量型腈水合酶的基因工程菌及其应用ZL 201510244547.0 一种比酶活和稳定性提高的融合型腈水合酶（分案）ZL 201510226690.7