· 2011-2012 美国WakeForest University医学院脂质研究中心 博士后 · 2001-2006 浙江大学材化学院 生物工程专业 博士 · 1997-2001 浙江大学化工系 生物化工专业 学士

· 2013 - 至 今 江南大学 食品学院 教授、博导 · 2007 - 2013 江南大学 食品学院 副教授、硕导 · 2006 -2007 江南大学 食品学院 校聘副教授

[1] 教育部新世纪优秀人才支持计划：ω-3多不饱和脂肪酸合成途径中关键脱饱和酶的催化特性解析及改良（No: CET-13-0831），50万，2014.01-2016.12，主持 [2] 江苏省“六大人才高峰”计划：产共轭亚油酸乳酸菌资源的挖掘与利用研究（NY-019），B类（10万），2015.01-2017.12，主持 [3] 国家自然科学基金重点项目：食品微生物的功能优化与调控的基础研究（No: 20836003），200万，2009.01-2012.12，参与 [4] 国家自然科学基金面上项目：高山被孢霉多不饱和脂肪酸合成途径中关键酶的底物选择特性和催化位点专一性研究（No:21276108），82万，2013.01-2016.12，主持 [5] 国家自然科学基金面上项目：高山被孢霉ω-6/ω-3脂肪酸代谢流调控“分子开关”的分子改造及其机制研究（No：31271812），77万，2013.01-2016.12，参与 [6] 国家自然科学基金青年基金：基因搜索结合无细胞功能表达筛选天然细菌素的研究（No：20706023），17万，2008.01-2010.12，主持 [7] 十二五“863”重点课题：重要食品酶微生物发酵生产与高效制备关键技术（No：2011AA100905），100万，2011.01-2015.12，子课题主持 [8] 十一五“863”自由探索课题：耐热β-半乳糖苷酶的分子改良及基因工程菌构建（No：2007AA10Z316），50万，2007.08-2010.10，主持 [9] 十一五“863”自由探索课题：枯草芽孢杆菌多适性食品级表达系统构建及分泌途径改造（No：2006AA10Z318），50万，2006.12-2010.10，参与 [10] 中央高校基本科研业务费专项重点项目：产油微生物高山被孢霉的脂质合成通路研究（No：JUSRP51320B），80万，2013.1-2015.12，主持 [11] 中央高校基本科研业务费专项面上项目：耐热乳糖酶的分子定向进化与性能改造研究(JUSRP11017)，8万，2010.07-2012.06，主持

2015年 [1] ChenH, Hao G, Wang L,Wang H, Gu Z, Liu L, Zhang H, Chen W*, Chen Y Q*. Identification of a criticaldeterminant that enables efficient fatty acid synthesis in oleaginous fungi. ScientificReports, 2015, 5: 11247. [2] YangB, Chen H*, Stanton C, Ross RP, Zhang H, Chen Y Q, Chen W*. Review of the roles of conjugated linoleic acidin health and disease, Journal of Functional Foods, 2015, 15: 314-325. [3] DongYN, Chen H*, Sun YH, Zhang H,Chen W. A differentially conserved residue (Ile42) of GH42 β-galactosidase fromGeobacillus stearothermophilus BgaB is involved in both catalysis and thermostability.J Dairy Sci. 2015; 98(4):2268-76 [4] Tang X, ChenH, Chen Y Q, Chen W, Garre V, Song Y*, Ratledge C. Comparison ofBiochemical Activities between High and Low Lipid-Producing Strains of Mucorcircinelloides: An Explanation for the High Oleaginicity of Strain WJ11[J].PLoS ONE, 2015, 10(6): e0128396. [5] Ye C, Xu N, Chen H, Chen YQ, Chen W, Liu L*. Reconstruction and analysisof a genome-scale metabolic model of the oleaginous fungus Mortierella alpina. BMCSyst Biol. 2015;9(1):1 2014年 [6] Wang G, Xia Y, Cui J, Gu Z, Song Y, Chen YQ,Chen H*, Zhang H, Chen W*.The roles of moonlighting proteins in Bacteria. Current Issues MolecularBiology. 2014, 16:15-22 [7] Hao G, ChenH*, Du K, Huang X, Song Y, Gu Z, Wang L, Zhang H, Chen W, Chen YQ.Increased fatty acid unsaturation and production of arachidonic acid byhomologous over-expression of the mitochondrial malic enzyme in Mortierellaalpina. Biotechnology Letters. 2014, 36( 9):1827-1834 [8] Hao G#, ChenH#, Wang L, Gu Z, Song Y, Zhang H, Chen W*, Chen YQ*. Role of MalicEnzyme during Fatty Acid Synthesis in the Oleaginous Fungus Mortierella alpina.Applied and Environmental Microbiology. 2014, 80(9):2672-2678. [9] Yang B, ChenH*, Gu Z, Tian F, Ross RP, Stanton C, Chen YQ, Chen W, Zhang H. Synthesisof conjugated linoleic acid by the linoleate isomerase complex in food-derivedlactobacilli. J Appl Microbiol. 2014;117(2):430-9 [10] Tang X, Zhang H, Chen H, Chen YQ, Chen W, Song Y*. Effects of 20 StandardAmino Acids on the Growth, Total Fatty Acids Production, and γ-Linolenic AcidYield in Mucor circinelloides. Curr Microbiol. 2014, 69(6):899-908. [11] Zhang H, Zhang L, Chen H, Chen YQ, Chen W, Song Y*, Ratledge C. Enhanced lipidaccumulation in the yeast Yarrowia lipolytica by over-expression of ATP:citratelyase from Mus musculus. J Biotechnol. 2014;192PA:78-84. [12] Wang G, Zhao Y, Tian F, Jin X，ChenH，Liu X，Zhang Q, Zhao J, Chen YQ, Zhang H, Chen W*. Screening of adhesive lactobacilli with antagonistic activity againstCampylobacter jejuni. Food Control. 2014, 44: 49-57 [13] Yang J, Hu X, Zhang H, Chen H, Kargbo MR, Zhao J, Song Y*, Chen YQ, Zhang H, Chen W*.Expression, Purification, and Characterization ofNADP(+)-Dependent Malic Enzymefrom the Oleaginous Fungus Mortierella Alpina. Applied Biochemistry andBiotechnology. 2014, 173(7): 1849-1857 2013年 [14] ChenH, Gu Z, Zhang H,Wang M, Chen W, Lowther WT, Chen YQ*: Expression and purification of integralmembrane fatty acid desaturases. PLoS ONE 2013, 8(3):e58139. [15] Wang M, ChenH*, Gu Z, Zhang H, Chen W, Chen YQ. Omega3 fatty acid desaturases frommicroorganisms: structure, function, evolution, and biotechnological use.Applied microbiology and biotechnology. 2013, 97:10255–10262 [16] Zhang B#, Chen H#, Li M, Gu Z, Song Y, Ratledge C, Chen YQ, Zhang H*,Chen W*. Genetic engineering of Yarrowia lipolytica for enhanced production oftrans-10, cis-12 conjugated linoleic acid. MICROBIAL CELL FACTORIES. 2013,12:70 [17] Yang B, ChenH*, Song Y, Chen Y, Zhang H, Chen W: Myosin-cross-reactive antigensfrom four different lactic acid bacteria are fatty acid hydratases.Biotechnology Letters 2013, 35(1):75-81. [18] Wang H, ChenH*, Hao G, Yang B, Feng Y, Wang Y, Feng L, Zhao J, Song Y, Zhang H, ChenY, Chen W*: Role of the Phenylalanine-Hydroxylating System in AromaticSubstance Degradation and Lipid Metabolism in the Oleaginous Fungus Mortierellaalpina. Applied and Environmental Microbiology 2013, 79(10):3225-3233. [19] Dong Y#, Wang L#, Gu Q, Chen H*, Liu X, Song Y, Chen W, Hagler A, Zhang H, Xu J*:Optimizing lactose hydrolysis by computer-guided modification of the catalyticsite of a wild-type enzyme. Molecular Diversity 2013, 17(2):371-382. [20] Wang G, ChenH, Zhang H, Song Y*, Chen W*. The Secretion of an IntrinsicallyDisordered Protein with Different Secretion Signals in Bacillus subtilis.Current Microbiology. 2013, 66:566–572 [21] Suburu J, Gu Z, Chen H, Chen W, Zhang H, Chen Y*. Fatty acid metabolism:Implications for diet, genetic variation, and disease. Food Biosci.2013;4:1-12. [22] Wang G, ChenH, Xia Y, Cui J, Gu Z, Song Y, Chen YQ, Zhang H*, Chen W*. How are theNon-classically Secreted Bacterial Proteins Released into the ExtracellularMilieu? Curr Microbiol. 2013, 67:688–695. [23] Zhang H, Zhang L, Chen H, Chen YQ, Ratledge C, Song Y*, Chen W. Regulatoryproperties of malic enzyme in the oleaginous yeast, Yarrowia lipolytica, andits non-involvement in lipid accumulation. Biotechnol Lett. 2013;35(12):2091-2098 [24] Gu Z, Suburu J, Chen H, Chen YQ*. Mechanisms of Omega-3 PolyunsaturatedFatty Acids in Prostate Cancer Prevention. BioMed research international. 2013,DOI: 10.1155/2013/824563 [25] Gu Z, Wu J, Wang S, Suburu J, Chen H, Thomas MJ, Shi L,Edwards IJ, Berquin IM, Chen YQ*. Polyunsaturated fatty acids affect thelocalization and signaling of PIP3/AKT in prostate cancer cells.CARCINOGENESIS. 2013, 34(9): 1968-1975 2012年以前 [26] ChenHQ, Yan X, Tian FW,Song YD, Chen YQ, Zhang H, Chen W*: Cloning, expression, and identification ofa novel class IIa bacteriocin in the Escherichia coli cell-free proteinexpression system. Biotechnology Letters 2012, 34(2):359-364. [27] ChenHQ, Tian FW, Li S,Xie Y, Zhang H, Chen W*: Cloning and heterologous expression of a bacteriocinsakacin P from Lactobacillus sakei in Escherichia coli. Applied Microbiologyand Biotechnology 2012, 94(4):1061-1068. [28] Zhang BX, Rong CC, Chen HQ, Song YD*, Zhang H, Chen W*: De novo synthesis oftrans-10, cis-12 conjugated linoleic acid in oleaginous yeast YarrowiaLipolytica. Microbial Cell Factories 2012, 11. [29] Wang L#, Chen W#, Feng Y, Ren Y, Gu ZN, Chen HQ, Wang HC, Thomas MJ,Zhang BX, Berquin IM et al: Genome Characterization of the Oleaginous FungusMortierella alpina. PLoS ONE 2011, 6(12). [30] Wang HC, Yang B, Hao GF, Feng Y, Chen HQ, Feng L, Zhao JX, ZhangH, Chen YQ, Wang L, Chen W*: Biochemical characterization of thetetrahydrobiopterin synthesis pathway in the oleaginous fungus Mortierellaalpina. Microbiology-Sgm 2011, 157:3059-3070. [31] Dong YN, Liu XM, Chen HQ, Xia Y, Zhang HP, Zhang H, Chen W*: Enhancement ofthe hydrolysis activity of beta-galactosidase from Geobacillusstearothermophilus by saturation mutagenesis. Journal of Dairy Science 2011,94(3):1176-1184. [32] Zhao JX, Yang LA, Gu ZN, Chen HQ, Tian FW, Chen YQ, ZhangH, Chen W*: Stabilization of the Single-Chain Fragment Variable by anInterdomain Disulfide Bond and Its Effect on Antibody Affinity. InternationalJournal of Molecular Sciences 2011, 12(1):1-11. [33] Xia Y, Zhao J, Chen H, Liu X, Wang Y, Tian F, Zhang HP, Zhang H, Chen W*:Extracellular secretion in Bacillus subtilis of a cytoplasmic thermostablebeta-galactosidase from Geobacillus stearothermophilus. Journal of DairyScience 2010, 93(7):2838-2845. [34] Cheikhyoussef A, Cheikhyoussef N, Chen HQ, Zhao JX, Tang J, ZhangH, Chen W*: Bifidin I - A new bacteriocin produced by Bifidobacterium infantisBCRC 14602: Purification and partial amino acid sequence. Food Control 2010,21(5):746-753. [35] Zhao JX, Dai XJ, Liu XM, Chen HQ, Tang J, Zhang H, Chen W*:Changes in microbial community during Chinese traditional soybean pastefermentation. International Journal of Food Science and Technology 2009,44(12):2526-2530. [36] Yang L, Ding H, Gu Z, Zhao J, Chen H, Tian F, Chen YQ, ZhangH, Chen W*. Selection of single chain fragment variables with direct coating ofaflatoxin B1 to enzyme-linked immunosorbent assay plates. J Agric Food Chem.2009;57(19):8927-32. [37] Chen W*, Chen H, Xia Y, Yang J, Zhao J, Tian F, Zhang HP, Zhang H:Immobilization of recombinant thermostable beta-galactosidase from Bacillusstearothermophilus for lactose hydrolysis in milk. Journal of Dairy Science2009, 92(2):491-498. [38] Cheikhyoussef A, Pogori N, Chen HQ, Tian FW, Chen W*, TangJ, Zhang H: Antimicrobial activity and partial characterization ofbacteriocin-like inhibitory substances (BLIS) produced by Bifidobacteriuminfantis BCRC 14602. Food Control 2009, 20(6):553-559. [39] Chen W*, Chen H, Xia Y, Zhao J, Tian F, Zhang H: Production,purification, and characterization of a potential thermostable galactosidasefor milk lactose hydrolysis from Bacillus stearothermophilus. Journal of DairyScience 2008, 91(5):1751-1758. [40] ChenHQ, Xu ZN*, Yin XF,Cen PL: Cloning and expression of the HIV protein in Escherichia coli cell-freesystem. Applied Microbiology and Biotechnology 2007, 77(2):347-354. [41] ChenHQ, Fan LM, Xu ZN*,Yin XF, Cen PL: Efficient production of soluble human beta-defensin-3-4 fusionproteins in Escherichia coli cell-free system. Process Biochemistry 2007,42(3):423-428. [42] Huang L, Xu Z*, Zhong Z, Peng L, Chen H, Cen P. Enhancedexpression and primary purification of soluble HBD3 fusion protein inEscherichia coli. Appl Biochem Biotechnol. 2007;142(2):139-47. [43] ChenHQ, Xu ZN*, Xu NZ,Cen PL: High-level expression of human beta-defensin-2 gene with rare codons inE-coli cell-free system. Protein and Peptide Letters 2006, 13(2):155-161. [44] Huang L, Wang JF, Zhong ZX, Peng L, Chen HQ, Xu ZN*, Cen PL:Production of bioactive human beta-defensin-3 in Escherichia coli by solublefusion expression. Biotechnology Letters 2006, 28(9):627-632. [45] ChenHQ, Xu ZN, Peng L,Fang XM, Yin XF, Xu NZ, Cen PL*: Recent advances in the research anddevelopment of human defensins. Peptides 2006, 27(4):931-940. [46] Xu ZN*, ChenHQ, Yin XF, Xu NZ, Cen PL: High-level expression of soluble humanbeta-defensin-2 fused with green fluorescent protein in Escherichia coli cell-freesystem. Applied Biochemistry and Biotechnology 2005, 127(1):53-62. [47] Chen HQ, Xu ZN*, Xu NZ, Cen PL: Efficient productionof a soluble fusion protein containing human beta-defensin-2 in E-colicell-free system. Journal of Biotechnology 2005, 115(3):307-315.

授权专利： [1] Haiqin Chen,Zhennan Gu, Hao Zhang, Wei Chen,Yuanda Song, Fengwei Tian, Jianxin Zhao,Yongquan Chen. An Omega-3 desaturase used in the biosynthesis of polyunsaturatedfatty acids (Application Number: US13646667，PatentNumber : US 8962276)（美国专利） [2] 陈卫，陈海琴，王光强，宋元达，顾震南，张灏，陈永泉.发明名称：利用非经典分泌蛋白质实现外源蛋白质的分泌表达 (ZL 201310245544.X) [3] 陈卫，张灏，张白曦，李敏，宋元达，陈海琴，赵建新，陈永泉，顾震南.基于基因工程策略优化耶氏解脂酵母重组菌合成共轭亚油酸 (ZL 201310120175.1) [4] 陈海琴，顾震南，张灏，陈卫，宋元达，田丰伟，赵建新，陈永泉. 一种用于多不饱和脂肪酸生物合成的脱饱和酶-ω3 Des (ZL 201210309215.2) [5] 陈卫，王光强，宋元达，陈海琴.利用一种新的转运信号来实现外源蛋白质的分泌表达 (ZL 201210476794.X) [6] 张灏，宋元达，张白曦，陈卫，陈海琴，刘晓明，赵建新.一种生产共轭亚油酸的重组酵母菌株及其用途（ZL 201210062130.9） [7] 陈卫，刘小鸣，田丰伟，张灏，赵建新，宋元达，刘文玉，范大明，王刚，张秋香，陈海琴. 一种植物乳杆菌N13及其用途 (ZL201210057822.4) [8] 陈卫，赵建新，田丰伟，张灏，王刚，张秋香，刘小鸣，陈海琴，宋元达，宁佳.一种乳酸片球菌CCFM7902及其用途 (ZL 201210054656.2) [9] 董艺凝，王领，陈卫，徐峻，张灏，陈海琴.一种耐热β-半乳糖苷酶突变体的构建 (ZL 201110170088.8) [10] 陈卫, 夏雨, 张灏,陈海琴,赵建新, 田丰伟,刘晓鸣.一种双精氨酸途径蛋白质分泌载体的构建方法及其应用（ZL 200810180274.8） [11] 田丰伟，陈卫，赵建新，张灏，张根义，张凤敏，陈海琴，戴小军，丁虎生.一种耐过氧化氢、清除自由基的抗氧化干酪乳杆菌及其用途 (ZL 200710145991.2) [12] 陈卫，张灏，赵建新，田丰伟，黄文利，丁虎生，戴小军，陈海琴.一种具有抗致突变活性、产胞外多糖的短乳杆菌及其用途 (ZL 200710145990.8) [13] 陈卫，张灏，田丰伟，赵建新，丁虎生，周凌华，钮晓燕，陈海琴.植物乳杆菌及用该菌生物制备共轭亚油酸的方法 (ZL 200610041191.1) 申请专利： [14] Wei Chen, HaiqinChen, Zhennan Gu, Hao Zhang, Yuanda Song, JianxinZhao, Fengwei Tian, Yongquan Chen. A delta-9 desaturase used in thebiosynthesis of polyunsaturated fatty acids (US13646668) (美国专利) [15] 陈卫，陈海琴，陈永泉，郝光飞，顾震南，赵建新，张灏，黄小云，杜凯，赵山山.一种通过同源重组敲除ura5基因的高山被孢霉尿嘧啶营养缺陷型及其构建方法(PCT/CN2014/072350)(PCT国际专利) [16] 陈永泉,陈卫，郝光飞，陈海琴，赵建新，顾震南，张灏，郝丹辉，赵山山.一种高山被孢霉重组基因表达系统及其构建方法和应用(PCT/CN2014/072839) (PCT国际专利) [17] 陈永泉，陈海琴，陈卫，代鹏，王鸿超，杨芹，赵建新，顾震南，张灏.一种高山被孢霉CCFM442菌株及其用途 (201510062057.9) [18] 陈海琴，陈卫，陈永泉，郝丹辉，郝光飞，杨波，张白曦，顾震南，张灏.一种异源表达亚油酸异构酶基因的重组高山被孢霉菌株及其构建方法(201510059567.0) [19] 陈海琴，陈永泉，陈卫，陈思，顾震南，赵建新，张灏，杨芹，王鸿超. 一种通过无细胞蛋白质合成系统表达脂肪酸脱饱和酶的方法(201410056249.4) [20] 陈永泉，陈卫，陈海琴，黄小云，顾震南，赵建新，张灏，杨芹，王鸿超. 一种过表达w3脱饱和酶基因的高山被孢霉基因工程菌株及其构建方法(201410087487.1) [21] 陈卫，张白曦，陈海琴，李敏，顾震南，赵建新，张秋香，刘小鸣，赵国忠，张灏，陈永泉.一种使用重组耶氏解脂酵母菌株生产含有共轭亚油酸的脂肪酸甲酯的方法(201410020846.1) [22] 陈卫，李敏，张白曦，陈海琴，赵建新，顾震南，田丰伟，王刚，张灏，陈永泉.一种使用含金属离子培养基生产含有共轭亚油酸的脂肪酸甲酯的方法 (201410021562.4) [23] 陈卫，陈海琴，李敏，张白曦，赵建新，顾震南，宋元达，田丰伟，杨芹，陈永泉，张灏.一种采用多次转化发酵法生产含有共轭亚油酸的脂肪酸甲酯的方法 (201410020823.0) [24] 陈永泉，陈卫，郝光飞，陈海琴，郝丹辉，赵山山，赵建新，顾震南，张灏.一种高山被孢霉重组基因表达系统及其构建方法和应用 (201310524221.4) [25] 陈卫，郝光飞，陈永泉，陈海琴，黄小云，杜凯，赵山山，张灏.一种通过同源重组敲除ura5基因的高山被孢霉尿嘧啶营养缺陷型及其构建方法(201310347934.8) [26] 陈卫，王光强，宋元达，陈海琴.利用鞭毛蛋白Hag的前50个氨基酸作为融合标签提高外源蛋白质在枯草芽孢杆菌中的表达量 (201210476792.0) [27] 陈海琴，陈卫，杨波，田丰伟，赵建新，宋元达，陈永泉，张灏. 一种利用毕赤酵母表达系统异源表达活性膜蛋白的方法 (201210414486.4) 陈卫，陈海琴，顾震南，张灏，宋元达，赵建新，田丰伟，陈永泉. 一种用于多不饱和脂肪酸生物合成的脱饱和酶-△9 Des (201210310769.4)